Human MAP3K15 ELISA Kit

Alternative name
Human apoptosis signal-regulating kinase 3 ELISA Kit;Human MAPK/ERK kinase kinase 15 ELISA Kit;Human MEK kinase 15 ELISA Kit;Human MEKK 15 ELISA Kit;Human ASK3 ELISA Kit;Human bA723P2.3 ELISA Kit;Human mitogen-activated protein kinase kinase kinase 15 ELISA Kit;
Catalog
E024727
species
Human
GeneMAP3K15
SpecificityThis assay has high sensitivity and excellent specificity for detection of MAP3K15. No significant cross-reactivity or interference between MAP3K15 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAP3K15 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman MAP3K15 ELISA Kit allows for the in vitro quantitative determination of MAP3K15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: MAP3K15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAP3K15 antibody and an MAP3K15-HRP conjugate. The assay sample and buffer are incubated together with MAP3K15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAP3K15 concentration since MAP3K15 from samples and MAP3K15-HRP conjugate compete for the anti-MAP3K15 antibody binding site. Since the number of sites is limited, as more sites are occupied by MAP3K15 from the sample, fewer sites are left to bind MAP3K15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAP3K15 concentration in each sample is interpolated from this standard curve.
Human Mitogen-activated protein kinase kinase kinase 15 Protein information
Uniprot ID M3K15_HUMAN
Uniprot AC Q6ZN16; A2AI49; A2AI50; A6NJ61; Q5JPR4; Q6ZMV3;
UniGene Hs.713701;
GeneID 389840
KEGG hsa:389840;
Human Mitogen-activated protein kinase kinase kinase 15 Protein SEQUENCE
SEQUENCE 1313 AA; 147437 MW; 60751706D6AEE3E9 CRC64;
MESGGGNAPA GALGAASESP QCPPPPGVEG AAGPAEPDGA AEGAAGGSGE
GESGGGPRRA LRAVYVRSES SQGGAAGGPE AGARQCLLRA CEAEGAHLTS
VPFGELDFGE TAVLDAFYDA DVAVVDMSDV SRQPSLFYHL GVRESFDMAN
NVILYHDTDA DTALSLKDMV TQKNTASSGN YYFIPYIVTP CADYFCCESD
AQRRASEYMQ PNWDNILGPL CMPLVDRFIS LLKDIHVTSC VYYKETLLND
IRKAREKYQG EELAKELARI KLRMDNTEVL TSDIIINLLL SYRDIQDYDA
MVKLVETLEM LPTCDLADQH NIKFHYAFAL NRRNSTGDRE KALQIMLQVL
QSCDHPGPDM FCLCGRIYKD IFLDSDCKDD TSRDSAIEWY RKGFELQSSL
YSGINLAVLL IVAGQQFETS LELRKIGVRL NSLLGRKGSL EKMNNYWDVG
QFFSVSMLAH DVGKAVQAAE RLFKLKPPVW YLRSLVQNLL LIRRFKKTII
EHSPRQERLN FWLDIIFEAT NEVTNGLRFP VLVIEPTKVY QPSYVSINNE
AEERTVSLWH VSPTEMKQMH EWNFTASSIK GISLSKFDER CCFLYVHDNS
DDFQIYFSTE EQCSRFFSLV KEMITNTAGS TVELEGETDG DTLEYEYDHD
ANGERVVLGK GTYGIVYAGR DLSNQVRIAI KEIPERDSRY SQPLHEEIAL
HKYLKHRNIV QYLGSVSENG YIKIFMEQVP GGSLSALLRS KWGPMKEPTI
KFYTKQILEG LKYLHENQIV HRDIKGDNVL VNTYSGVVKI SDFGTSKRLA
GVNPCTETFT GTLQYMAPEI IDQGPRGYGA PADIWSLGCT IIEMATSKPP
FHELGEPQAA MFKVGMFKIH PEIPEALSAE ARAFILSCFE PDPHKRATTA
ELLREGFLRQ VNKGKKNRIA FKPSEGPRGV VLALPTQGEP MATSSSEHGS
VSPDSDAQPD ALFERTRAPR HHLGHLLSVP DESSALEDRG LASSPEDRDQ
GLFLLRKDSE RRAILYKILW EEQNQVASNL QECVAQSSEE LHLSVGHIKQ
IIGILRDFIR SPEHRVMATT ISKLKVDLDF DSSSISQIHL VLFGFQDAVN
KILRNHLIRP HWMFAMDNII RRAVQAAVTI LIPELRAHFE PTCETEGVDK
DMDEAEEGYP PATGPGQEAQ PHQQHLSLQL GELRQETNRL LEHLVEKERE
YQNLLRQTLE QKTQELYHLQ LKLKSNCITE NPAGPYGQRT DKELIDWLRL
QGADAKTIEK IVEEGYTLSD ILNEITKEDL RYLRLRGGLL CRLWSAVSQY
RRAQEASETK DKA
UCSC uc004czj.3; human. [Q6ZN16-1];

Category

Human MAP3K15 ELISA Kit

Human apoptosis signal-regulating kinase 3 ELISA Kit;Human MAPK/ERK kinase kinase 15 ELISA Kit;Human MEK kinase 15 ELISA Kit;Human MEKK 15 ELISA Kit;Human ASK3 ELISA Kit;Human bA723P2.3 ELISA Kit;Human mitogen-activated protein kinase kinase kinase 15 ELISA Kit;