Human MAP4K4 ELISA Kit

Alternative name
Human HPK/GCK-like kinase HGK ELISA Kit;Human MAPK/ERK kinase kinase kinase 4 ELISA Kit;Human MEK kinase kinase 4 ELISA Kit;Human MEKKK 4 ELISA Kit;Human nck-interacting kinase ELISA Kit;Human HGK ELISA Kit;Human KIAA0687 ELISA Kit;Human NIK ELISA Kit;Human FLH21957 ELISA Kit;Human HEL-S-31 ELISA Kit;Human MEKKK4 ELISA Kit;Human mitogen-activated protein kinase kinase kinase kinase 4 ELISA Kit;Human Ste20 group protein kinase HGK ELISA Kit;Human epididymis secretory protein Li 31 ELISA Kit;Human hepatocyte progenitor kinase-like/germinal center kinase-like kinase ELISA Kit;
Catalog
E024758
species
Human
GeneMAP4K4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MAP4K4. No significant cross-reactivity or interference between Human MAP4K4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity5.8 pg/ml
Intended UseHuman MAP4K4 ELISA Kit allows for the in vitro quantitative determination of MAP4K4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP4K4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP4K4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP4K4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP4K4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Mitogen-activated protein kinase kinase kinase kinase 4 Protein information
Uniprot ID M4K4_HUMAN
Uniprot AC O95819; O75172; Q9NST7;
UniGene Hs.701013;
GeneID 9448
KEGG hsa:9448;
Human Mitogen-activated protein kinase kinase kinase kinase 4 Protein SEQUENCE
SEQUENCE 1239 AA; 142101 MW; 8FBBE2F9ABEEC757 CRC64;
MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL
AAIKVMDVTE DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ
LWLVMEFCGA GSITDLVKNT KGNTLKEDWI AYISREILRG LAHLHIHHVI
HRDIKGQNVL LTENAEVKLV DFGVSAQLDR TVGRRNTFIG TPYWMAPEVI
ACDENPDATY DYRSDLWSCG ITAIEMAEGA PPLCDMHPMR ALFLIPRNPP
PRLKSKKWSK KFFSFIEGCL VKNYMQRPST EQLLKHPFIR DQPNERQVRI
QLKDHIDRTR KKRGEKDETE YEYSGSEEEE EEVPEQEGEP SSIVNVPGES
TLRRDFLRLQ QENKERSEAL RRQQLLQEQQ LREQEEYKRQ LLAERQKRIE
QQKEQRRRLE EQQRREREAR RQQEREQRRR EQEEKRRLEE LERRRKEEEE
RRRAEEEKRR VEREQEYIRR QLEEEQRHLE VLQQQLLQEQ AMLLECRWRE
MEEHRQAERL QRQLQQEQAY LLSLQHDHRR PHPQHSQQPP PPQQERSKPS
FHAPEPKAHY EPADRAREVE DRFRKTNHSS PEAQSKQTGR VLEPPVPSRS
ESFSNGNSES VHPALQRPAE PQVPVRTTSR SPVLSRRDSP LQGSGQQNSQ
AGQRNSTSIE PRLLWERVEK LVPRPGSGSS SGSSNSGSQP GSHPGSQSGS
GERFRVRSSS KSEGSPSQRL ENAVKKPEDK KEVFRPLKPA DLTALAKELR
AVEDVRPPHK VTDYSSSSEE SGTTDEEDDD VEQEGADEST SGPEDTRAAS
SLNLSNGETE SVKTMIVHDD VESEPAMTPS KEGTLIVRQT QSASSTLQKH
KSSSSFTPFI DPRLLQISPS SGTTVTSVVG FSCDGMRPEA IRQDPTRKGS
VVNVNPTNTR PQSDTPEIRK YKKRFNSEIL CAALWGVNLL VGTESGLMLL
DRSGQGKVYP LINRRRFQQM DVLEGLNVLV TISGKKDKLR VYYLSWLRNK
ILHNDPEVEK KQGWTTVGDL EGCVHYKVVK YERIKFLVIA LKSSVEVYAW
APKPYHKFMA FKSFGELVHK PLLVDLTVEE GQRLKVIYGS CAGFHAVDVD
SGSVYDIYLP THIQCSIKPH AIIILPNTDG MELLVCYEDE GVYVNTYGRI
TKDVVLQWGE MPTSVAYIRS NQTMGWGEKA IEIRSVETGH LDGVFMHKRA
QRLKFLCERN DKVFFASVRS GGSSQVYFMT LGRTSLLSW
UCSC uc002tbg.4; human. [O95819-1];

Category

Human MAP4K4 ELISA Kit