Human SMAD1 ELISA Kit

Alternative name
Human MAD homolog 1 ELISA Kit;Human mothers against DPP homolog 1 ELISA Kit;Human JV4-1 ELISA Kit;Human Mad-related protein 1 ELISA Kit;Human SMAD family member 1 ELISA Kit;Human SMAD 1 ELISA Kit;Human Smad1 ELISA Kit;Human hSMAD1 ELISA Kit;Human Transforming growth factor-beta-signaling protein 1 ELISA Kit;Human BSP-1 ELISA Kit;Human BSP1 ELISA Kit;Human MADH1 ELISA Kit;Human MADR1 ELISA Kit;Human JV41 ELISA Kit;Human mothers against decapentaplegic homolog 1 ELISA Kit;Human MAD, mothers against decapentaplegic homolog 1 ELISA Kit;Human SMAD, mothers against DPP homolog 1 ELISA Kit;Human TGF-beta signaling protein 1 ELISA Kit;Human transforming growth factor-beta signaling protein 1 ELISA Kit;
Catalog
E024914
species
Human
GeneSMAD1
SpecificityThis assay has high sensitivity for detection of Smad1. 100% cross-reactivity of Smad1 was observed among human, mouse, rat and bovine.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Smad1 is typically less than 0.132ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman SMAD1 ELISA Kit allows for the in vitro quantitative determination of SMAD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Smad1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Smad1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Smad1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Smad1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Mothers against decapentaplegic homolog 1 Protein information
Uniprot ID SMAD1_HUMAN
Uniprot AC Q15797; A8KAJ0; D3DNZ9; Q16636; Q9UFT8;
UniGene Hs.604588;
GeneID 4086
KEGG hsa:4086;
Human Mothers against decapentaplegic homolog 1 Protein SEQUENCE
SEQUENCE 465 AA; 52260 MW; 2DD34B7F434DBC7E CRC64;
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE
LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH
HELKPLECCE FPFGSKQKEV CINPYHYKRV ESPVLPPVLV PRHSEYNPQH
SLLAQFRNLG QNEPHMPLNA TFPDSFQQPN SHPFPHSPNS SYPNSPGSSS
STYPHSPTSS DPGSPFQMPA DTPPPAYLPP EDPMTQDGSQ PMDTNMMAPP
LPSEINRGDV QAVAYEEPKH WCSIVYYELN NRVGEAFHAS STSVLVDGFT
DPSNNKNRFC LGLLSNVNRN STIENTRRHI GKGVHLYYVG GEVYAECLSD
SSIFVQSRNC NYHHGFHPTT VCKIPSGCSL KIFNNQEFAQ LLAQSVNHGF
ETVYELTKMC TIRMSFVKGW GAEYHRQDVT STPCWIEIHL HGPLQWLDKV
LTQMGSPHNP ISSVS
UCSC uc003ikc.4; human. [Q15797-1];

Category

Human SMAD1 ELISA Kit