Human MST1 ELISA Kit

Alternative name
Human Macrophage stimulatory protein ELISA Kit;Human macrophage-stimulating protein ELISA Kit;Human MSP ELISA Kit;Human D3F15S2 ELISA Kit;Human DNF15S2 ELISA Kit;Human HGFL ELISA Kit;Human NF15S2 ELISA Kit;Human macrophage stimulating 1 ELISA Kit;Human hepatocyte growth factor-like protein ELISA Kit;Human hepatocyte growth factor-like protein homolog ELISA Kit;Human macrophage stimulating 1 (hepatocyte growth factor-like) ELISA Kit;
Catalog
E024985
species
Human
GeneMST1
SpecificityNatural and recombinant human MSP
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman MST1 ELISA Kit allows for the in vitro quantitative determination of MST1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human MSP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MSP has been precoated onto 96-well plates. Standards(NSO, Q19-G711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MSP amount of sample captured in plate. Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Human Hepatocyte growth factor-like protein beta chain Protein information
Uniprot ID HGFL_HUMAN
Uniprot AC P26927; A6NLA3; A8MSX3; Q13350; Q14870; Q6GTN4;
UniGene Hs.349110; Hs.512587;
GeneID 4485
KEGG hsa:4485;
Human Hepatocyte growth factor-like protein beta chain Protein SEQUENCE
SEQUENCE 711 AA; 80320 MW; 2E4B3C7D4AA9B566 CRC64;
MGWLPLLLLL TQCLGVPGQR SPLNDFQVLR GTELQHLLHA VVPGPWQEDV
ADAEECAGRC GPLMDCRAFH YNVSSHGCQL LPWTQHSPHT RLRRSGRCDL
FQKKDYVRTC IMNNGVGYRG TMATTVGGLP CQAWSHKFPN DHKYTPTLRN
GLEENFCRNP DGDPGGPWCY TTDPAVRFQS CGIKSCREAA CVWCNGEEYR
GAVDRTESGR ECQRWDLQHP HQHPFEPGKF LDQGLDDNYC RNPDGSERPW
CYTTDPQIER EFCDLPRCGS EAQPRQEATT VSCFRGKGEG YRGTANTTTA
GVPCQRWDAQ IPHQHRFTPE KYACKDLREN FCRNPDGSEA PWCFTLRPGM
RAAFCYQIRR CTDDVRPQDC YHGAGEQYRG TVSKTRKGVQ CQRWSAETPH
KPQFTFTSEP HAQLEENFCR NPDGDSHGPW CYTMDPRTPF DYCALRRCAD
DQPPSILDPP DQVQFEKCGK RVDRLDQRRS KLRVVGGHPG NSPWTVSLRN
RQGQHFCGGS LVKEQWILTA RQCFSSCHMP LTGYEVWLGT LFQNPQHGEP
SLQRVPVAKM VCGPSGSQLV LLKLERSVTL NQRVALICLP PEWYVVPPGT
KCEIAGWGET KGTGNDTVLN VALLNVISNQ ECNIKHRGRV RESEMCTEGL
LAPVGACEGD YGGPLACFTH NCWVLEGIII PNRVCARSRW PAVFTRVSVF
VDWIHKVMRL G

Category

Human MST1 ELISA Kit