Human MUC20 ELISA Kit

Alternative name
Human MUC-20 ELISA Kit;Human KIAA1359 ELISA Kit;Human mucin 20, cell surface associated ELISA Kit;Human mucin-20 ELISA Kit;Human transmembrane mucin MUC20S ELISA Kit;
Catalog
E025037
species
Human
GeneMUC20
SpecificityThis assay has high sensitivity and excellent specificity for detection of MUC20. No significant cross-reactivity or interference between MUC20 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MUC20 is typically less than 0.064ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman MUC20 ELISA Kit allows for the in vitro quantitative determination of MUC20 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MUC20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MUC20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MUC20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MUC20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Mucin-20 Protein information
Uniprot ID MUC20_HUMAN
Uniprot AC Q8N307; Q6UX97; Q76I83; Q76I85; Q86ST8; Q8NBY6; Q96KA1; Q9P2I8;
UniGene Hs.308992;
GeneID 200958
KEGG hsa:200958;
Human Mucin-20 Protein SEQUENCE
SEQUENCE 709 AA; 71982 MW; DD344A6014D39172 CRC64;
MGCLWGLALP LFFFCWEVGV SGSSAGPSTR RADTAMTTDD TEVPAMTLAP
GHAALETQTL SAETSSRAST PAGPIPEAET RGAKRISPAR ETRSFTKTSP
NFMVLIATSV ETSAASGSPE GAGMTTVQTI TGSDPREAIF DTLCTDDSSE
EAKTLTMDIL TLAHTSTEAK GLSSESSASS DSPHPVITPS RASESSASSD
GPHPVITPSR ASESSASSDG PHPVITPSRA SESSASSDGP HPVITPSRAS
ESSASSDGPH PVITPSRASE SSASSDGPHP VITPSRASES SASSDGPHPV
ITPSRASESS ASSDGPHPVI TPSRASESSA SSDGPHPVIT PSRASESSAS
SDGLHPVITP SRASESSASS DGPHPVITPS RASESSASSD GPHPVITPSW
SPGSDVTLLA EALVTVTNIE VINCSITEIE TTTSSIPGAS DTDLIPTEGV
KASSTSDPPA LPDSTEAKPH ITEVTASAET LSTAGTTESA APDATVGTPL
PTNSATEREV TAPGATTLSG ALVTVSRNPL EETSALSVET PSYVKVSGAA
PVSIEAGSAV GKTTSFAGSS ASSYSPSEAA LKNFTPSETP TMDIATKGPF
PTSRDPLPSV PPTTTNSSRG TNSTLAKITT SAKTTMKPPT ATPTTARTRP
TTDVSAGENG GFLLLRLSVA SPEDLTDPRV AERLMQQLHR ELHAHAPHFQ
VSLLRVRRG
UCSC uc032soi.2; human. [Q8N307-1];

Category

Human MUC20 ELISA Kit

Human MUC-20 ELISA Kit;Human KIAA1359 ELISA Kit;Human mucin 20, cell surface associated ELISA Kit;Human mucin-20 ELISA Kit;Human transmembrane mucin MUC20S ELISA Kit;