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Alternative name
Mucin-1 ELISA KIT; Breast carcinoma-associated antigen DF3 ELISA KIT; Cancer antigen 15-3 ELISA KIT; CA 15-3MUC1 ELISA KIT; PUM ELISA KIT; MUC-1 ELISA KIT; CA 15-3 ELISA KIT; KL-6 ELISA KIT; PUM ELISA KIT; PEM ELISA KIT; EMA ELISA KIT; MUC1-NT ELISA KIT; MUC1-alpha ELISA KIT; MUC1-beta ELISA KIT
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Catalog
E025064
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species
Human
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GeneMUC-1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human MUC-1. No significant cross-reactivity or interference between Human MUC-1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.094ng/ml
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Intended UseHuman MUC-1 ELISA Kit allows for the in vitro quantitative determination of MUC-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human MUC-1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MUC-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MUC-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human MUC-1 can be calculated.
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