Human MLH1 ELISA Kit

Alternative name
Human MutL protein homolog 1 ELISA Kit;Human COCA2 ELISA Kit;Human FCC2 ELISA Kit;Human HNPCC ELISA Kit;Human HNPCC2 ELISA Kit;Human hMLH1 ELISA Kit;Human mutL homolog 1 ELISA Kit;Human DNA mismatch repair protein Mlh1 ELISA Kit;Human mutL homolog 1, colon cancer, nonpolyposis type 2 ELISA Kit;
Catalog
E025199
species
Human
GeneMLH1
SpecificityThis assay has high sensitivity and excellent specificity for detection of MLH1). No significant cross-reactivity or interference between MLH1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human MLH1 is typically less than 0.053ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman MLH1 ELISA Kit allows for the in vitro quantitative determination of MLH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MLH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MLH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MLH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA mismatch repair protein Mlh1 Protein information
Uniprot ID MLH1_HUMAN
Uniprot AC P40692; B4DI13; B4DQ11; E9PCU2;
UniGene Hs.195364;
GeneID 4292
KEGG hsa:4292;
Human DNA mismatch repair protein Mlh1 Protein SEQUENCE
SEQUENCE 756 AA; 84601 MW; C9231FC406C2CA20 CRC64;
MSFVAGVIRR LDETVVNRIA AGEVIQRPAN AIKEMIENCL DAKSTSIQVI
VKEGGLKLIQ IQDNGTGIRK EDLDIVCERF TTSKLQSFED LASISTYGFR
GEALASISHV AHVTITTKTA DGKCAYRASY SDGKLKAPPK PCAGNQGTQI
TVEDLFYNIA TRRKALKNPS EEYGKILEVV GRYSVHNAGI SFSVKKQGET
VADVRTLPNA STVDNIRSIF GNAVSRELIE IGCEDKTLAF KMNGYISNAN
YSVKKCIFLL FINHRLVEST SLRKAIETVY AAYLPKNTHP FLYLSLEISP
QNVDVNVHPT KHEVHFLHEE SILERVQQHI ESKLLGSNSS RMYFTQTLLP
GLAGPSGEMV KSTTSLTSSS TSGSSDKVYA HQMVRTDSRE QKLDAFLQPL
SKPLSSQPQA IVTEDKTDIS SGRARQQDEE MLELPAPAEV AAKNQSLEGD
TTKGTSEMSE KRGPTSSNPR KRHREDSDVE MVEDDSRKEM TAACTPRRRI
INLTSVLSLQ EEINEQGHEV LREMLHNHSF VGCVNPQWAL AQHQTKLYLL
NTTKLSEELF YQILIYDFAN FGVLRLSEPA PLFDLAMLAL DSPESGWTEE
DGPKEGLAEY IVEFLKKKAE MLADYFSLEI DEEGNLIGLP LLIDNYVPPL
EGLPIFILRL ATEVNWDEEK ECFESLSKEC AMFYSIRKQY ISEESTLSGQ
QSEVPGSIPN SWKWTVEHIV YKALRSHILP PKHFTEDGNI LQLANLPDLY
KVFERC
UCSC uc003cgl.4; human. [P40692-1];

Category

Human MLH1 ELISA Kit

Human MutL protein homolog 1 ELISA Kit;Human COCA2 ELISA Kit;Human FCC2 ELISA Kit;Human HNPCC ELISA Kit;Human HNPCC2 ELISA Kit;Human hMLH1 ELISA Kit;Human mutL homolog 1 ELISA Kit;Human DNA mismatch repair protein Mlh1 ELISA Kit;Human mutL homolog 1, colon cancer, nonpolyposis type 2 ELISA Kit;