Human MCL1 ELISA Kit

Alternative name
Human bcl-2-like protein 3 ELISA Kit;Human bcl2-L-3 ELISA Kit;Human bcl-2-related protein EAT/mcl1 ELISA Kit;Human mcl1/EAT ELISA Kit;Human BCL2L3 ELISA Kit;Human EAT ELISA Kit;Human MCL1-ES ELISA Kit;Human MCL1L ELISA Kit;Human MCL1S ELISA Kit;Human Mcl-1 ELISA Kit;Human TM ELISA Kit;Human BCL2 family apoptosis regulator ELISA Kit;Human induced myeloid leukemia cell differentiation protein Mcl-1 ELISA Kit;Human myeloid cell leukemia 1 ELISA Kit;Human myeloid cell leukemia ES ELISA Kit;Human myeloid cell leukemia sequence 1 (BCL2-related) ELISA Kit;
Catalog
E025295
species
Human
GeneMCL1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.114ng/mL.
Intended UseHuman MCL1 ELISA Kit allows for the in vitro quantitative determination of MCL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Induced myeloid leukemia cell differentiation protein Mcl-1 Protein information
Uniprot ID MCL1_HUMAN
Uniprot AC Q9UHR8; Q9UHR9; Q9UNJ1;
UniGene Hs.632486;
GeneID 4170
KEGG hsa:4170;
Human Induced myeloid leukemia cell differentiation protein Mcl-1 Protein SEQUENCE
SEQUENCE 350 AA; 37337 MW; D85821AC59275F1F CRC64;
MFGLKRNAVI GLNLYCGGAG LGAGSGGATR PGGRLLATEK EASARREIGG
GEAGAVIGGS AGASPPSTLT PDSRRVARPP PIGAEVPDVT ATPARLLFFA
PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV LPLLELVGES
GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG
RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM
IHVFSDGVTN WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR
TKRDWLVKQR GWDGFVEFFH VEDLEGGIRN VLLAFAGVAG VGAGLAYLIR
UCSC uc001euz.4; human. [Q07820-1];

Category

Human MCL1 ELISA Kit