Human MNDA ELISA Kit

Alternative name
Human PYHIN3 ELISA Kit;Human myeloid cell nuclear differentiation antigen ELISA Kit;
Catalog
E025296
species
Human
GeneMNDA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman MNDA ELISA Kit allows for the in vitro quantitative determination of MNDA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MNDA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MNDA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MNDA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MNDA can be calculated.
Human Myeloid cell nuclear differentiation antigen Protein information
Uniprot ID MNDA_HUMAN
Uniprot AC P41218;
UniGene Hs.153837;
GeneID 4332
KEGG hsa:4332;
Human Myeloid cell nuclear differentiation antigen Protein SEQUENCE
SEQUENCE 407 AA; 45836 MW; F4943C38033C5A83 CRC64;
MVNEYKKILL LKGFELMDDY HFTSIKSLLA YDLGLTTKMQ EEYNRIKITD
LMEKKFQGVA CLDKLIELAK DMPSLKNLVN NLRKEKSKVA KKIKTQEKAP
VKKINQEEVG LAAPAPTARN KLTSEARGRI PVAQKRKTPN KEKTEAKRNK
VSQEQSKPPG PSGASTSAAV DHPPLPQTSS STPSNTSFTP NQETQAQRQV
DARRNVPQND PVTVVVLKAT APFKYESPEN GKSTMFHATV ASKTQYFHVK
VFDINLKEKF VRKKVITISD YSECKGVMEI KEASSVSDFN QNFEVPNRII
EIANKTPKIS QLYKQASGTM VYGLFMLQKK SVHKKNTIYE IQDNTGSMDV
VGSGKWHNIK CEKGDKLRLF CLQLRTVDRK LKLVCGSHSF IKVIKAKKNK
EGPMNVN

Category

Human MNDA ELISA Kit

Human PYHIN3 ELISA Kit;Human myeloid cell nuclear differentiation antigen ELISA Kit;