-
Alternative name
Protein ENL ELISA KIT; YEATS domain-containing protein 1MLLT1 ELISA KIT; ENL ELISA KIT; LTG19 ELISA KIT; YEATS1 ELISA KIT
-
Catalog
E025320
-
species
Human
-
GeneMLL1
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of MLL1. No significant cross-reactivity or interference between MLL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MLL1 and all the analogues, therefore, cross reaction may still exist in some cases.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.1 ng/mL.
-
Intended UseHuman MLL1 ELISA Kit allows for the in vitro quantitative determination of MLL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Categories/FamilyEpigenetics and Nuclear Signaling
-
Product Description
specificalIntended Uses: This MLL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MLL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||MLL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MLL1 antibody and an MLL1-HRP conjugate. The assay sample and buffer are incubated together with MLL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MLL1 concentration since MLL1 from samples and MLL1-HRP conjugate compete for the anti-MLL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MLL1 from the sample, fewer sites are left to bind MLL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLL1 concentration in each sample is interpolated from this standard curve.
-