Human MYOC ELISA Kit

Alternative name
Human myocilin 55 kDa subunit ELISA Kit;Human Trabecular meshwork-induced glucocorticoid response protein ELISA Kit;Human Myocilin 20 kDa N-terminal fragment ELISA Kit;Human Myocilin 35 kDa N-terminal fragment ELISA Kit;Human GLC1A ELISA Kit;Human TIGR ELISA Kit;Human GPOA ELISA Kit;Human JOAG ELISA Kit;Human JOAG1 ELISA Kit;Human myocilin ELISA Kit;Human juvenile-onset open-angle glaucoma 1 ELISA Kit;Human mutated trabecular meshwork-induced glucocorticoid response protein ELISA Kit;Human myocilin trabecular meshwork inducible glucocorticoid response protein ELISA Kit;Human trabecular meshwork inducible glucocorticoid response protein ELISA Kit;
Catalog
E025357
species
Human
GeneMYOC
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myocilin (MYOC). No significant cross-reactivity or interference between Myocilin (MYOC) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.26ng/mL.
Intended UseHuman MYOC ELISA Kit allows for the in vitro quantitative determination of MYOC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myocilin (MYOC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myocilin (MYOC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myocilin (MYOC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myocilin (MYOC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Myocilin, C-terminal fragment Protein information
Uniprot ID MYOC_HUMAN
Uniprot AC Q99972; B2RD84; O00620; Q7Z6Q9;
UniGene Hs.436037;
GeneID 4653
KEGG hsa:4653;
Human Myocilin, C-terminal fragment Protein SEQUENCE
SEQUENCE 504 AA; 56972 MW; 9588C04F1D227623 CRC64;
MRFFCARCCS FGPEMPAVQL LLLACLVWDV GARTAQLRKA NDQSGRCQYT
FSVASPNESS CPEQSQAMSV IHNLQRDSST QRLDLEATKA RLSSLESLLH
QLTLDQAARP QETQEGLQRE LGTLRRERDQ LETQTRELET AYSNLLRDKS
VLEEEKKRLR QENENLARRL ESSSQEVARL RRGQCPQTRD TARAVPPGSR
EVSTWNLDTL AFQELKSELT EVPASRILKE SPSGYLRSGE GDTGCGELVW
VGEPLTLRTA ETITGKYGVW MRDPKPTYPY TQETTWRIDT VGTDVRQVFE
YDLISQFMQG YPSKVHILPR PLESTGAVVY SGSLYFQGAE SRTVIRYELN
TETVKAEKEI PGAGYHGQFP YSWGGYTDID LAVDEAGLWV IYSTDEAKGA
IVLSKLNPEN LELEQTWETN IRKQSVANAF IICGTLYTVS SYTSADATVN
FAYDTGTGIS KTLTIPFKNR YKYSSMIDYN PLEKKLFAWD NLNMVTYDIK
LSKM
UCSC uc001ghu.4; human.;

Category

Human MYOC ELISA Kit