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Human MYH11 ELISA Kit

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  • Alternative name

    Human myosin heavy chain 11 ELISA Kit;Human myosin heavy chain, smooth muscle isoform ELISA Kit;Human SMMHC ELISA Kit;Human KIAA0866 ELISA Kit;Human AAT4 ELISA Kit;Human FAA4 ELISA Kit;Human SMHC ELISA Kit;Human myosin-11 ELISA Kit;Human myosin, heavy chain 11, smooth muscle ELISA Kit;Human myosin, heavy polypeptide 11, smooth muscle ELISA Kit;
  • Catalog
    E025406
  • species
    Human
  • GeneMYH11
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MYH-II. No significant cross-reactivity or interference between MYH-II and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MYH-II and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman MYH11 ELISA Kit allows for the in vitro quantitative determination of MYH11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the assay: MYH-II ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYH-II antibody and an MYH-II-HRP conjugate. The assay sample and buffer are incubated together with MYH-II-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYH-II concentration since MYH-II from samples and MYH-II-HRP conjugate compete for the anti-MYH-II antibody binding site. Since the number of sites is limited, as more sites are occupied by MYH-II from the sample, fewer sites are left to bind MYH-II-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYH-II concentration in each sample is interpolated from this standard curve.
  • Human Myosin-11 Protein information
  • Uniprot ID MYH11_HUMAN
  • Uniprot AC P35749; D2JYH7; O00396; O94944; P78422; Q3MIV8; Q3MNF0; Q3MNF1;
  • UniGene Hs.460109;
  • GeneID 4629
  • KEGG hsa:4629;
  • Human Myosin-11 Protein SEQUENCE

  • SEQUENCE 1972 AA; 227339 MW; 67665BB2AECE1277 CRC64;

    MAQKGQLSDD EKFLFVDKNF INSPVAQADW AAKRLVWVPS EKQGFEAASI

    KEEKGDEVVV ELVENGKKVT VGKDDIQKMN PPKFSKVEDM AELTCLNEAS

    VLHNLRERYF SGLIYTYSGL FCVVVNPYKH LPIYSEKIVD MYKGKKRHEM

    PPHIYAIADT AYRSMLQDRE DQSILCTGES GAGKTENTKK VIQYLAVVAS

    SHKGKKDTSI TGELEKQLLQ ANPILEAFGN AKTVKNDNSS RFGKFIRINF

    DVTGYIVGAN IETYLLEKSR AIRQARDERT FHIFYYMIAG AKEKMRSDLL

    LEGFNNYTFL SNGFVPIPAA QDDEMFQETV EAMAIMGFSE EEQLSILKVV

    SSVLQLGNIV FKKERNTDQA SMPDNTAAQK VCHLMGINVT DFTRSILTPR

    IKVGRDVVQK AQTKEQADFA VEALAKATYE RLFRWILTRV NKALDKTHRQ

    GASFLGILDI AGFEIFEVNS FEQLCINYTN EKLQQLFNHT MFILEQEEYQ

    REGIEWNFID FGLDLQPCIE LIERPNNPPG VLALLDEECW FPKATDKSFV

    EKLCTEQGSH PKFQKPKQLK DKTEFSIIHY AGKVDYNASA WLTKNMDPLN

    DNVTSLLNAS SDKFVADLWK DVDRIVGLDQ MAKMTESSLP SASKTKKGMF

    RTVGQLYKEQ LGKLMTTLRN TTPNFVRCII PNHEKRSGKL DAFLVLEQLR

    CNGVLEGIRI CRQGFPNRIV FQEFRQRYEI LAANAIPKGF MDGKQACILM

    IKALELDPNL YRIGQSKIFF RTGVLAHLEE ERDLKITDVI MAFQAMCRGY

    LARKAFAKRQ QQLTAMKVIQ RNCAAYLKLR NWQWWRLFTK VKPLLQVTRQ

    EEEMQAKEDE LQKTKERQQK AENELKELEQ KHSQLTEEKN LLQEQLQAET

    ELYAEAEEMR VRLAAKKQEL EEILHEMEAR LEEEEDRGQQ LQAERKKMAQ

    QMLDLEEQLE EEEAARQKLQ LEKVTAEAKI KKLEDEILVM DDQNNKLSKE

    RKLLEERISD LTTNLAEEEE KAKNLTKLKN KHESMISELE VRLKKEEKSR

    QELEKLKRKL EGDASDFHEQ IADLQAQIAE LKMQLAKKEE ELQAALARLD

    DEIAQKNNAL KKIRELEGHI SDLQEDLDSE RAARNKAEKQ KRDLGEELEA

    LKTELEDTLD STATQQELRA KREQEVTVLK KALDEETRSH EAQVQEMRQK

    HAQAVEELTE QLEQFKRAKA NLDKNKQTLE KENADLAGEL RVLGQAKQEV

    EHKKKKLEAQ VQELQSKCSD GERARAELND KVHKLQNEVE SVTGMLNEAE

    GKAIKLAKDV ASLSSQLQDT QELLQEETRQ KLNVSTKLRQ LEEERNSLQD

    QLDEEMEAKQ NLERHISTLN IQLSDSKKKL QDFASTVEAL EEGKKRFQKE

    IENLTQQYEE KAAAYDKLEK TKNRLQQELD DLVVDLDNQR QLVSNLEKKQ

    RKFDQLLAEE KNISSKYADE RDRAEAEARE KETKALSLAR ALEEALEAKE

    ELERTNKMLK AEMEDLVSSK DDVGKNVHEL EKSKRALETQ MEEMKTQLEE

    LEDELQATED AKLRLEVNMQ ALKGQFERDL QARDEQNEEK RRQLQRQLHE

    YETELEDERK QRALAAAAKK KLEGDLKDLE LQADSAIKGR EEAIKQLRKL

    QAQMKDFQRE LEDARASRDE IFATAKENEK KAKSLEADLM QLQEDLAAAE

    RARKQADLEK EELAEELASS LSGRNALQDE KRRLEARIAQ LEEELEEEQG

    NMEAMSDRVR KATQQAEQLS NELATERSTA QKNESARQQL ERQNKELRSK

    LHEMEGAVKS KFKSTIAALE AKIAQLEEQV EQEAREKQAA TKSLKQKDKK

    LKEILLQVED ERKMAEQYKE QAEKGNARVK QLKRQLEEAE EESQRINANR

    RKLQRELDEA TESNEAMGRE VNALKSKLRR GNETSFVPSR RSGGRRVIEN

    ADGSEEETDT RDADFNGTKA SE

  • UCSC uc002ddv.4; human. [P35749-1];



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