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Human MYH6 ELISA Kit

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  • Alternative name

    Human myosin heavy chain 6 ELISA Kit;Human myosin heavy chain, cardiac muscle alpha isoform ELISA Kit;Human myHC-alpha ELISA Kit;Human MYHCA ELISA Kit;Human ASD3 ELISA Kit;Human CMD1EE ELISA Kit;Human CMH14 ELISA Kit;Human MYHC ELISA Kit;Human SSS3 ELISA Kit;Human alpha-MHC ELISA Kit;Human myosin-6 ELISA Kit;Human myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1) ELISA Kit;
  • Catalog
    E025433
  • species
    Human
  • GeneMYH6
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). No significant cross-reactivity or interference between Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.125ng/mL.
  • Intended UseHuman MYH6 ELISA Kit allows for the in vitro quantitative determination of MYH6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Myosin-6 Protein information
  • Uniprot ID MYH6_HUMAN
  • Uniprot AC P13533; A2RTX1; D9YZU2; Q13943; Q14906; Q14907;
  • UniGene Hs.278432;
  • GeneID 4624
  • KEGG hsa:4624;
  • Human Myosin-6 Protein SEQUENCE

  • SEQUENCE 1939 AA; 223735 MW; 424211CC048C8004 CRC64;

    MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEFVKAK

    ILSREGGKVI AETENGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA

    VLFNLKERYA AWMIYTYSGL FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA

    PPHIFSISDN AYQYMLTDRE NQSILITGES GAGKTVNTKR VIQYFASIAA

    IGDRGKKDNA NANKGTLEDQ IIQANPALEA FGNAKTVRND NSSRFGKFIR

    IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD

    MLLVTNNPYD YAFVSQGEVS VASIDDSEEL MATDSAFDVL GFTSEEKAGV

    YKLTGAIMHY GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL

    CHPRVKVGNE YVTKGQSVQQ VYYSIGALAK AVYEKMFNWM VTRINATLET

    KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN FTNEKLQQFF NHHMFVLEQE

    EYKKEGIEWT FIDFGMDLQA CIDLIEKPMG IMSILEEECM FPKATDMTFK

    AKLYDNHLGK SNNFQKPRNI KGKQEAHFSL IHYAGTVDYN ILGWLEKNKD

    PLNETVVALY QKSSLKLMAT LFSSYATADT GDSGKSKGGK KKGSSFQTVS

    ALHRENLNKL MTNLRTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV

    LEGIRICRKG FPNRILYGDF RQRYRILNPV AIPEGQFIDS RKGTEKLLSS

    LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD ERLSRIITRM QAQARGQLMR

    IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP LLKSAETEKE

    MATMKEEFGR IKETLEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL

    NDAEERCDQL IKNKIQLEAK VKEMNERLED EEEMNAELTA KKRKLEDECS

    ELKKDIDDLE LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA

    LQEAHQQALD DLQVEEDKVN SLSKSKVKLE QQVDDLEGSL EQEKKVRMDL

    ERAKRKLEGD LKLTQESIMD LENDKLQLEE KLKKKEFDIN QQNSKIEDEQ

    VLALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD LSRELEEISE

    RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD

    SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV

    SRTLEDQANE YRVKLEEAQR SLNDFTTQRA KLQTENGELA RQLEEKEALI

    SQLTRGKLSY TQQMEDLKRQ LEEEGKAKNA LAHALQSARH DCDLLREQYE

    EETEAKAELQ RVLSKANSEV AQWRTKYETD AIQRTEELEE AKKKLAQRLQ

    DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA AAALDKKQRN

    FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF

    KRENKNLQEE ISDLTEQLGE GGKNVHELEK VRKQLEVEKL ELQSALEEAE

    ASLEHEEGKI LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHQRVVDSLQ

    TSLDAETRSR NEVLRVKKKM EGDLNEMEIQ LSHANRMAAE AQKQVKSLQS

    LLKDTQIQLD DAVRANDDLK ENIAIVERRN NLLQAELEEL RAVVEQTERS

    RKLAEQELIE TSERVQLLHS QNTSLINQKK KMESDLTQLQ SEVEEAVQEC

    RNAEEKAKKA ITDAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD

    EAEQIALKGG KKQLQKLEAR VRELEGELEA EQKRNAESVK GMRKSERRIK

    ELTYQTEEDK KNLLRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK

    VQHELDEAEE RADIAESQVN KLRAKSRDIG AKQKMHDEE

  • UCSC uc001wjv.3; human.;



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