Human MYL3 ELISA Kit

Alternative name
Human cardiac myosin light chain 1 ELISA Kit;Human CMLC1 ELISA Kit;Human Myosin light chain 1, slow-twitch muscle B/ventricular isoform ELISA Kit;Human MLC1SB ELISA Kit;Human ventricular myosin alkali light chain ELISA Kit;Human ventricular myosin light chain 1 ELISA Kit;Human VLCl ELISA Kit;Human ventricular/slow twitch myosin alkali light chain ELISA Kit;Human MLC-lV/sb ELISA Kit;Human CMH8 ELISA Kit;Human MLC1V ELISA Kit;Human VLC1 ELISA Kit;Human myosin light chain 3 ELISA Kit;Human myosin light chain 1, slow-twitch muscle B/ventricular isoform ELISA Kit;Human myosin, light chain 3, alkali; ventricular, skeletal, slow ELISA Kit;Human myosin, light polypeptide 3, alkali; ventricular, skeletal, slow ELISA Kit;
Catalog
E025471
species
Human
GeneMYL3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). No significant cross-reactivity or interference between Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 28pg/mL.
Intended UseHuman MYL3 ELISA Kit allows for the in vitro quantitative determination of MYL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 3, Alkali, Ventricular, Slow Skeletal (MYL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Myosin light chain 3 Protein information
Uniprot ID MYL3_HUMAN
Uniprot AC P08590; B2R534; Q9NRS8;
UniGene Hs.517939;
GeneID 4634
KEGG hsa:4634;
Human Myosin light chain 3 Protein SEQUENCE
SEQUENCE 195 AA; 21932 MW; 306CF328841729DD CRC64;
MAPKKPEPKK DDAKAAPKAA PAPAPPPEPE RPKEVEFDAS KIKIEFTPEQ
IEEFKEAFML FDRTPKCEMK ITYGQCGDVL RALGQNPTQA EVLRVLGKPR
QEELNTKMMD FETFLPMLQH ISKNKDTGTY EDFVEGLRVF DKEGNGTVMG
AELRHVLATL GERLTEDEVE KLMAGQEDSN GCINYEAFVK HIMSS
UCSC uc003cql.2; human.;

Category

Human MYL3 ELISA Kit