Human PGLYRP2 ELISA Kit

Alternative name
Human peptidoglycan recognition protein 2 ELISA Kit;Human peptidoglycan recognition protein long ELISA Kit;Human PGRP-L ELISA Kit;Human PGLYRPL ELISA Kit;Human PGRPL ELISA Kit;Human HMFT0141 ELISA Kit;Human TAGL-like ELISA Kit;Human tagL ELISA Kit;Human tagL-alpha ELISA Kit;Human tagl-beta ELISA Kit;Human N-acetylmuramoyl-L-alanine amidase ELISA Kit;Human peptidoglycan recognition protein L ELISA Kit;
Catalog
E025574
species
Human
GenePGLYRP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PGLYRP2. No significant cross-reactivity or interference between PGLYRP2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGLYRP2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman PGLYRP2 ELISA Kit allows for the in vitro quantitative determination of PGLYRP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This PGLYRP2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGLYRP2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PGLYRP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-PGLYRP2 antibody and an PGLYRP2-HRP conjugate. The assay sample and buffer are incubated together with PGLYRP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGLYRP2 concentration since PGLYRP2 from samples and PGLYRP2-HRP conjugate compete for the anti-PGLYRP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGLYRP2 from the sample, fewer sites are left to bind PGLYRP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGLYRP2 concentration in each sample is interpolated from this standard curve.
Human N-acetylmuramoyl-L-alanine amidase Protein information
Uniprot ID PGRP2_HUMAN
Uniprot AC Q96PD5; A8K050; A8K8C7; B2RMZ2; B7ZM33; Q68CK1; Q96N74; Q9UC60;
UniGene Hs.282244;
GeneID 114770
KEGG hsa:114770;
Human N-acetylmuramoyl-L-alanine amidase Protein SEQUENCE
SEQUENCE 576 AA; 62217 MW; 73EA8713DC54F85A CRC64;
MAQGVLWILL GLLLWSDPGT ASLPLLMDSV IQALAELEQK VPAAKTRHTA
SAWLMSAPNS GPHNRLYHFL LGAWSLNATE LDPCPLSPEL LGLTKEVARH
DVREGKEYGV VLAPDGSTVA VEPLLAGLEA GLQGRRVINL PLDSMAAPWE
TGDTFPDVVA IAPDVRATSS PGLRDGSPDV TTADIGANTP DATKGCPDVQ
ASLPDAKAKS PPTMVDSLLA VTLAGNLGLT FLRGSQTQSH PDLGTEGCWD
QLSAPRTFTL LDPKASLLTM AFLNGALDGV ILGDYLSRTP EPRPSLSHLL
SQYYGAGVAR DPGFRSNFRR QNGAALTSAS ILAQQVWGTL VLLQRLEPVH
LQLQCMSQEQ LAQVAANATK EFTEAFLGCP AIHPRCRWGA APYRGRPKLL
QLPLGFLYVH HTYVPAPPCT DFTRCAANMR SMQRYHQDTQ GWGDIGYSFV
VGSDGYVYEG RGWHWVGAHT LGHNSRGFGV AIVGNYTAAL PTEAALRTVR
DTLPSCAVRA GLLRPDYALL GHRQLVRTDC PGDALFDLLR TWPHFTATVK
PRPARSVSKR SRREPPPRTL PATDLQ
UCSC uc002nbf.5; human. [Q96PD5-1];

Category

Human PGLYRP2 ELISA Kit