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Human APEH ELISA Kit

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  • Alternative name

    Human AARE ELISA Kit;Human acyl-peptide hydrolase ELISA Kit;Human APH ELISA Kit;Human acylaminoacyl-peptidase ELISA Kit;Human oxidized protein hydrolase ELISA Kit;Human OPH ELISA Kit;Human D3F15S2 ELISA Kit;Human D3S48E ELISA Kit;Human DNF15S2 ELISA Kit;Human ACPH ELISA Kit;Human acylaminoacyl-peptide hydrolase ELISA Kit;Human acylamino-acid-releasing enzyme ELISA Kit;Human N-acylaminoacyl-peptide hydrolase ELISA Kit;
  • Catalog
    E025641
  • species
    Human
  • GeneAPEH
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human APEH. No significant cross-reactivity or interference between human APEH and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human APEH is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman APEH ELISA Kit allows for the in vitro quantitative determination of APEH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APEH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APEH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APEH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APEH bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Acylamino-acid-releasing enzyme Protein information
  • Uniprot ID ACPH_HUMAN
  • Uniprot AC P13798; Q9BQ33; Q9P0Y2;
  • UniGene Hs.517969;
  • GeneID 327
  • KEGG hsa:327;
  • Human Acylamino-acid-releasing enzyme Protein SEQUENCE

  • SEQUENCE 732 AA; 81225 MW; A2C370516324D851 CRC64;

    MERQVLLSEP EEAAALYRGL SRQPALSAAC LGPEVTTQYG GQYRTVHTEW

    TQRDLERMEN IRFCRQYLVF HDGDSVVFAG PAGNSVETRG ELLSRESPSG

    TMKAVLRKAG GTGPGEEKQF LEVWEKNRKL KSFNLSALEK HGPVYEDDCF

    GCLSWSHSET HLLYVAEKKR PKAESFFQTK ALDVSASDDE IARLKKPDQA

    IKGDQFVFYE DWGENMVSKS IPVLCVLDVE SGNISVLEGV PENVSPGQAF

    WAPGDAGVVF VGWWHEPFRL GIRFCTNRRS ALYYVDLIGG KCELLSDDSL

    AVSSPRLSPD QCRIVYLQYP SLIPHHQCSQ LCLYDWYTKV TSVVVDVVPR

    QLGENFSGIY CSLLPLGCWS ADSQRVVFDS AQRSRQDLFA VDTQVGTVTS

    LTAGGSGGSW KLLTIDQDLM VAQFSTPSLP PTLKVGFLPS AGKEQSVLWV

    SLEEAEPIPD IHWGIRVLQP PPEQENVQYA GLDFEAILLQ PGSPPDKTQV

    PMVVMPHGGP HSSFVTAWML FPAMLCKMGF AVLLVNYRGS TGFGQDSILS

    LPGNVGHQDV KDVQFAVEQV LQEEHFDASH VALMGGSHGG FISCHLIGQY

    PETYRACVAR NPVINIASML GSTDIPDWCV VEAGFPFSSD CLPDLSVWAE

    MLDKSPIRYI PQVKTPLLLM LGQEDRRVPF KQGMEYYRAL KTRNVPVRLL

    LYPKSTHALS EVEVESDSFM NAVLWLRTHL GS

  • UCSC uc003cxf.4; human.;



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