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Human ASAH2 ELISA Kit

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  • Alternative name

    Human N-CDase ELISA Kit;Human NCDase ELISA Kit;Human acylsphingosine deacylase 2 ELISA Kit;Human BCDase ELISA Kit;Human LCDase ELISA Kit;Human hCD ELISA Kit;Human N-acylsphingosine amidohydrolase 2 ELISA Kit;Human non-lysosomal ceramidase ELISA Kit;Human HNAC1 ELISA Kit;Human neutral ceramidase ELISA Kit;Human N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 ELISA Kit;Human mitochondrial ceramidase ELISA Kit;Human neutral/alkaline ceramidase ELISA Kit;
  • Catalog
    E025649
  • species
    Human
  • GeneASAH2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Acylsphingosine Amidohydrolase 2 (ASAH2). No significant cross-reactivity or interference between N-Acylsphingosine Amidohydrolase 2 (ASAH2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.31ng/mL.
  • Intended UseHuman ASAH2 ELISA Kit allows for the in vitro quantitative determination of ASAH2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acylsphingosine Amidohydrolase 2 (ASAH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acylsphingosine Amidohydrolase 2 (ASAH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Acylsphingosine Amidohydrolase 2 (ASAH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neutral ceramidase soluble form Protein information
  • Uniprot ID ASAH2_HUMAN
  • Uniprot AC Q9NR71; Q3KNU1; Q5SNT7; Q5SZP6; Q5SZP7; Q5T1D5; Q71ME6;
  • UniGene Hs.512645;
  • GeneID 56624
  • KEGG hsa:56624;
  • Human Neutral ceramidase soluble form Protein SEQUENCE

  • SEQUENCE 780 AA; 85516 MW; D2BD7947B022A619 CRC64;

    MAKRTFSNLE TFLIFLLVMM SAITVALLSL LFITSGTIEN HKDLGGHFFS

    TTQSPPATQG STAAQRSTAT QHSTATQSST ATQTSPVPLT PESPLFQNFS

    GYHIGVGRAD CTGQVADINL MGYGKSGQNA QGILTRLYSR AFIMAEPDGS

    NRTVFVSIDI GMVSQRLRLE VLNRLQSKYG SLYRRDNVIL SGTHTHSGPA

    GYFQYTVFVI ASEGFSNQTF QHMVTGILKS IDIAHTNMKP GKIFINKGNV

    DGVQINRSPY SYLQNPQSER ARYSSNTDKE MIVLKMVDLN GDDLGLISWF

    AIHPVSMNNS NHLVNSDNVG YASYLLEQEK NKGYLPGQGP FVAAFASSNL

    GDVSPNILGP RCINTGESCD NANSTCPIGG PSMCIAKGPG QDMFDSTQII

    GRAMYQRAKE LYASASQEVT GPLASAHQWV DMTDVTVWLN STHASKTCKP

    ALGYSFAAGT IDGVGGLNFT QGKTEGDPFW DTIRDQILGK PSEEIKECHK

    PKPILLHTGE LSKPHPWHPD IVDVQIITLG SLAITAIPGE FTTMSGRRLR

    EAVQAEFASH GMQNMTVVIS GLCNVYTHYI TTYEEYQAQR YEAASTIYGP

    HTLSAYIQLF RNLAKAIATD TVANLSRGPE PPFFKQLIVP LIPSIVDRAP

    KGRTFGDVLQ PAKPEYRVGE VAEVIFVGAN PKNSVQNQTH QTFLTVEKYE

    ATSTSWQIVC NDASWETRFY WHKGLLGLSN ATVEWHIPDT AQPGIYRIRY

    FGHNRKQDIL KPAVILSFEG TSPAFEVVTI

  • UCSC uc001jjd.4; human. [Q9NR71-1];



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