Human SGSH ELISA Kit

Alternative name
Human sulfoglucosamine sulfamidase ELISA Kit;Human sulphamidase ELISA Kit;Human HSS ELISA Kit;Human MPS3A ELISA Kit;Human SFMD ELISA Kit;Human N-sulfoglucosamine sulfohydrolase ELISA Kit;Human N-sulphoglucosamine sulphohydrolase ELISA Kit;Human heparan sulfate sulfatase ELISA Kit;Human mucopolysaccharidosis type IIIA ELISA Kit;
Catalog
E025686
species
Human
GeneSGSH
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Sulfoglucosamine Sulfohydrolase (SGSH). No significant cross-reactivity or interference between N-Sulfoglucosamine Sulfohydrolase (SGSH) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.054ng/mL.
Intended UseHuman SGSH ELISA Kit allows for the in vitro quantitative determination of SGSH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Sulfoglucosamine Sulfohydrolase (SGSH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Sulfoglucosamine Sulfohydrolase (SGSH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Sulfoglucosamine Sulfohydrolase (SGSH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Sulfoglucosamine Sulfohydrolase (SGSH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human N-sulphoglucosamine sulphohydrolase Protein information
Uniprot ID SPHM_HUMAN
Uniprot AC P51688; A8K5E2;
UniGene Hs.31074;
GeneID 6448
KEGG hsa:6448;
Human N-sulphoglucosamine sulphohydrolase Protein SEQUENCE
SEQUENCE 502 AA; 56695 MW; 90C5CDAB4DCC3808 CRC64;
MSCPVPACCA LLLVLGLCRA RPRNALLLLA DDGGFESGAY NNSAIATPHL
DALARRSLLF RNAFTSVSSC SPSRASLLTG LPQHQNGMYG LHQDVHHFNS
FDKVRSLPLL LSQAGVRTGI IGKKHVGPET VYPFDFAYTE ENGSVLQVGR
NITRIKLLVR KFLQTQDDRP FFLYVAFHDP HRCGHSQPQY GTFCEKFGNG
ESGMGRIPDW TPQAYDPLDV LVPYFVPNTP AARADLAAQY TTVGRMDQGV
GLVLQELRDA GVLNDTLVIF TSDNGIPFPS GRTNLYWPGT AEPLLVSSPE
HPKRWGQVSE AYVSLLDLTP TILDWFSIPY PSYAIFGSKT IHLTGRSLLP
ALEAEPLWAT VFGSQSHHEV TMSYPMRSVQ HRHFRLVHNL NFKMPFPIDQ
DFYVSPTFQD LLNRTTAGQP TGWYKDLRHY YYRARWELYD RSRDPHETQN
LATDPRFAQL LEMLRDQLAK WQWETHDPWV CAPDGVLEEK LSPQCQPLHN
EL
UCSC uc002jxz.5; human.;

Category

Human SGSH ELISA Kit