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Alternative name
Sodium/bile acid cotransporter ELISA KIT; Cell growth-inhibiting gene 29 protein ELISA KIT; Na(+)/bile acid cotransporter ELISA KIT; Na(+)/taurocholate transport protein ELISA KIT; Sodium/taurocholate cotransporting polypeptide ELISA KIT; Solute carrier family 10 member 1SLC10A1 ELISA KIT; NTCP ELISA KIT
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Catalog
E025715
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species
Human
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GeneNTCP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Na+ Taurocholate Cotransporting Polypeptide (NTCP).
No significant cross-reactivity or interference between Na+ Taurocholate Cotransporting Polypeptide (NTCP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.27ng/mL.
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Intended UseHuman NTCP ELISA Kit allows for the in vitro quantitative determination of NTCP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na+ Taurocholate Cotransporting Polypeptide (NTCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Na+ Taurocholate Cotransporting Polypeptide (NTCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na+ Taurocholate Cotransporting Polypeptide (NTCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Na+ Taurocholate Cotransporting Polypeptide (NTCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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