Human NOX5 ELISA Kit

Alternative name
Human NADPH oxidase 5 ELISA Kit;Human NADPH oxidase, EF-hand calcium binding domain 5 ELISA Kit;
Catalog
E025830
species
Human
GeneNOX5
SpecificityThis assay has high sensitivity and excellent specificity for detection of human NOX5. No significant cross-reactivity or interference between human NOX5 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NOX5 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman NOX5 ELISA Kit allows for the in vitro quantitative determination of NOX5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOX5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human NADPH oxidase 5 Protein information
Uniprot ID NOX5_HUMAN
Uniprot AC Q96PJ9; Q9H6E0; Q9HAM8;
UniGene Hs.657932;
GeneID 79400
KEGG hsa:79400;
Human NADPH oxidase 5 Protein SEQUENCE
SEQUENCE 765 AA; 86439 MW; 96B57225BF919682 CRC64;
MNTSGDPAQT GPEGCRGTMS AEEDARWLRW VTQQFKTIAG EDGEISLQEF
KAALHVKESF FAERFFALFD SDRSGTITLQ ELQEALTLLI HGSPMDKLKF
LFQVYDIDVC ARQGASAGTE WGAGAGPHWA SSPLGTGSGS IDPDELRTVL
QSCLRESAIS LPDEKLDQLT LALFESADAD GNGAITFEEL RDELQRFPGV
MENLTISAAH WLTAPAPRPR PRRPRQLTRA YWHNHRSQLF CLATYAGLHV
LLFGLAASAH RDLGASVMVA KGCGQCLNFD CSFIAVLMLR RCLTWLRATW
LAQVLPLDQN IQFHQLMGYV VVGLSLVHTV AHTVNFVLQA QAEASPFQFW
ELLLTTRPGI GWVHGSASPT GVALLLLLLL MFICSSSCIR RSGHFEVFYW
THLSYLLVWL LLIFHGPNFW KWLLVPGILF FLEKAIGLAV SRMAAVCIME
VNLLPSKVTH LLIKRPPFFH YRPGDYLYLN IPTIARYEWH PFTISSAPEQ
KDTIWLHIRS QGQWTNRLYE SFKASDPLGR GSKRLSRSVT MRKSQRSSKG
SEILLEKHKF CNIKCYIDGP YGTPTRRIFA SEHAVLIGAG IGITPFASIL
QSIMYRHQKR KHTCPSCQHS WIEGVQDNMK LHKVDFIWIN RDQRSFEWFV
SLLTKLEMDQ AEEAQYGRFL ELHMYMTSAL GKNDMKAIGL QMALDLLANK
EKKDSITGLQ TRTQPGRPDW SKVFQKVAAE KKGKVQVFFC GSPALAKVLK
GHCEKFGFRF FQENF
UCSC uc002arp.3; human. [Q96PH1-1];

Category

Human NOX5 ELISA Kit

Human NADPH oxidase 5 ELISA Kit;Human NADPH oxidase, EF-hand calcium binding domain 5 ELISA Kit;