Human NECTIN4 ELISA Kit

Alternative name
Human Ig superfamily receptor LNIR ELISA Kit;Human nectin cell adhesion molecule 4 ELISA Kit;Human poliovirus receptor-related protein 4 ELISA Kit;Human EDSS1 ELISA Kit;Human LNIR ELISA Kit;Human PRR4 ELISA Kit;Human PVRL4 ELISA Kit;Human nectin-4 ELISA Kit;Human nectin 4 ELISA Kit;Human poliovirus receptor-related 4 ELISA Kit;
Catalog
E025907
species
Human
GeneNECTIN4
SpecificityNatural and recombinant Human Nectin-4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
Intended UseHuman NECTIN4 ELISA Kit allows for the in vitro quantitative determination of NECTIN4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: Human Nectin-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Nectin-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: G32-S349) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Nectin-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Nectin-4 amount of sample captured in plate. Background/Introduction: Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
Human Processed poliovirus receptor-related protein 4 Protein information
Uniprot ID NECT4_HUMAN
Uniprot AC Q96NY8; B4DQW3; Q96K15;
UniGene Hs.492490; Hs.732297;
GeneID 81607
KEGG hsa:81607;
Human Processed poliovirus receptor-related protein 4 Protein SEQUENCE
SEQUENCE 510 AA; 55454 MW; DCF5E1D794F227FA CRC64;
MPLSLGAEMW GPEAWLLLLL LLASFTGRCP AGELETSDVV TVVLGQDAKL
PCFYRGDSGE QVGQVAWARV DAGEGAQELA LLHSKYGLHV SPAYEGRVEQ
PPPPRNPLDG SVLLRNAVQA DEGEYECRVS TFPAGSFQAR LRLRVLVPPL
PSLNPGPALE EGQGLTLAAS CTAEGSPAPS VTWDTEVKGT TSSRSFKHSR
SAAVTSEFHL VPSRSMNGQP LTCVVSHPGL LQDQRITHIL HVSFLAEASV
RGLEDQNLWH IGREGAMLKC LSEGQPPPSY NWTRLDGPLP SGVRVDGDTL
GFPPLTTEHS GIYVCHVSNE FSSRDSQVTV DVLDPQEDSG KQVDLVSASV
VVVGVIAALL FCLLVVVVVL MSRYHRRKAQ QMTQKYEEEL TLTRENSIRR
LHSHHTDPRS QPEESVGLRA EGHPDSLKDN SSCSVMSEEP EGRSYSTLTT
VREIETQTEL LSPGSGRAEE EEDQDEGIKQ AMNHFVQENG TLRAKPTGNG
IYINGRGHLV
UCSC uc001fxo.3; human. [Q96NY8-1];

Category

Human NECTIN4 ELISA Kit