Human N4BP2 ELISA Kit

Alternative name
Human N4BP2 ELISA Kit;Human BCL-3-binding protein ELISA Kit;Human B3BP ELISA Kit;Human KIAA1413 ELISA Kit;Human NEDD4 binding protein 2 ELISA Kit;Human NEDD4-binding protein 2 ELISA Kit;
Catalog
E025910
species
Human
GeneN4BP2
SpecificityThis assay has high sensitivity and excellent specificity for detection of N4BP2. No significant cross-reactivity or interference between N4BP2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of N4BP2 is typically less than 0.118ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
Intended UseHuman N4BP2 ELISA Kit allows for the in vitro quantitative determination of N4BP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to N4BP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N4BP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N4BP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N4BP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human NEDD4-binding protein 2 Protein information
Uniprot ID N4BP2_HUMAN
Uniprot AC Q86UW6; A0AVR3; Q9NVK2; Q9P2D4;
UniGene Hs.391463; Hs.736026;
GeneID 55728
KEGG hsa:55728;
Human NEDD4-binding protein 2 Protein SEQUENCE
SEQUENCE 1770 AA; 198801 MW; 9A49FAB19C1BD56D CRC64;
MPRRRKNLGG NPFRKTANPK EVVVSSVASR EEPTTTLPSM GETKVDQEEL
FTSISEIFSD LDPDVVYLML SECDFKVENA MDCLLELSAT DTKIEESSSQ
SFVASENQVG AAESKIMEKR PEEESEDSKM DSFLDMQLTE DLDSLIQNAF
EKLNSSPDDQ VYSFLPSQDV NSFNDSSEFI NPDSSNMTPI FSTQNMNLNG
ENLENSGSTL SLNPLPSHSV LNESKCFIKD NTLALESNYP EDSLLSSSLN
VASDSIAGCS SLNQKQKELL ESECVEAQFS EAPVDLDASE PQACLNLPGL
DLPGTGGDQK STRVSDVFLP SEGFNFKPHK HPELPTKGKD VSYCPVLAPL
PLLLPPPPPP PMWNPMIPAF DLFQGNHGFV APVVTTAAHW RSVNYTFPPS
VISHTSPTKV WRNKDGTSAY QVQETPVSQV VRKKTSYVGL VLVLLRGLPG
SGKSFLARTL QEDNPSGVIL STDDYFYING QYQFDVKYLG EAHEWNQNRA
KEAFEKKISP IIIDNTNLQA WEMKPYVALS QKHKYKVLFR EPDTWWKFKP
KELARRNIHG VSKEKITRML EHYQRFVSVP IIMSSSVPEK IERIELCAYS
CEDRSTSPRD DEDIISEKEE NILSLSLKHL EFTEEKNLDV TKETMLPENV
AYLSNADLNK RRKEISDMNP SIQSALILET PHMYFSDSES KLQATDKSEN
EQIEMVAVKG YSKTDTDSSM ERVSPSTCCS ENNQEDCDLA NSGPLQNEKS
SPGEIVEERA TVTKKAFGKQ KSKSTLEKFP RHELSNFVGD WPVDKTIGQR
TKRNRKTEKT SSVQSDKKYN YPQSHKLVNS VSVNTDCVQQ RGSPHESVED
GRKSQCDDAS EPLNSYKYDA YKNIDKNSFN IMGDWPSSDS LAQREHRSRM
PKTGLSEPNL EIGTNDKMNE ISLSTAHEAC WGTSSQKLKT LGSSNLGSSE
MLLSEMTCES QTCLSKKSHG QHTSLPLTFT NSAPTVSGVV EPQTLAECQE
QMPKRDPGKE VGMCTQTEPQ DFALLWKIEK NKISISDSIK VLTGRLDGFK
PKVFNINTKS DVQEAIPYRV MYDKSTFVEE SELTSADESE NLNILCKLFG
SFSLEALKDL YERCNKDIIW ATSLLLDSET KLCEDTEFEN FQKSCDGSQI
GPFSLGLNLK EIISQRGTLE NSNSPVPEFS HGIGISNADS QSTCDAERGN
SEQAEMRAVT PENHESMTSI FPSAAVGLKN NNDILPNSQE ELLYSSKQSF
PGILKATTPK DMSETEKNLV VTETGDNIHS PSHFSDIFNF VSSTSNLELN
EEIYFTDSLE IKRNENFPKD YVKFSDEEEF MNEDEKEMKE ILMAGSSLSA
GVSGEDKTEI LNPTPAMAKS LTIDCLELAL PPELAFQLNE LFGPVGIDSG
SLTVEDCVVH IDLNLAKVIH EKWKESVMER QRQEEVSCGK FMQDPSLVGH
TGLDNPEQKS SQRTGKKLLK TLTASEMLPL LDHWNTQTKK VSLREIMSEE
IALQEKHNLK RETLMFEKDC ATKLKEKQLF KIFPAINQNF LVDIFKDHNY
SLEHTVQFLN CVLEGDPVKT VVAQEFVHQN ENVTSHTGQK SKEKKPKKLK
ETEETPSELS FQDFEYPDYD DYRAEAFLHQ QKRMECYSKA KEAYRIGKKN
VATFYAQQGT LHEQKMKEAN HLAAIEIFEK VNASLLPQNV LDLHGLHVDE
ALEHLMRVLE KKTEEFKQNG GKPYLSVITG RGNHSQGGVA RIKPAVIKYL
ISHSFRFSEI KPGCLKVMLK
UCSC uc003guy.5; human. [Q86UW6-1];

Category

Human N4BP2 ELISA Kit

Human N4BP2 ELISA Kit;Human BCL-3-binding protein ELISA Kit;Human B3BP ELISA Kit;Human KIAA1413 ELISA Kit;Human NEDD4 binding protein 2 ELISA Kit;Human NEDD4-binding protein 2 ELISA Kit;