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Human NEDD8 ELISA Kit

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  • Alternative name

    Human neddylin ELISA Kit;Human Neural precursor cell expressed developmentally down-regulated protein 8 ELISA Kit;Human NEDD-8 ELISA Kit;Human ubiquitin-like protein Nedd8 ELISA Kit;Human neural precursor cell expressed, developmentally down-regulated 8 ELISA Kit;Human NEDD8 ELISA Kit;
  • Catalog
    E026049
  • species
    Human
  • GeneNEDD8
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NEDD8. No significant cross-reactivity or interference between human NEDD8 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NEDD8 is typically less than 9.77 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman NEDD8 ELISA Kit allows for the in vitro quantitative determination of NEDD8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NEDD8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NEDD8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEDD8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEDD8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human NEDD8 Protein information
  • Uniprot ID NEDD8_HUMAN
  • Uniprot AC Q15843; Q3SXN8; Q6LES6;
  • UniGene Hs.531064;
  • GeneID 4738
  • KEGG hsa:4738;
  • Human NEDD8 Protein SEQUENCE

  • SEQUENCE 81 AA; 9072 MW; DC2FE102BE4725D2 CRC64;

    MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM

    NDEKTAADYK ILGGSVLHLV LALRGGGGLR Q

  • UCSC uc001wnn.3; human.;



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