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Human NET1 ELISA Kit

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  • Alternative name

    Human Proto-oncogene p65 Net1 ELISA Kit;Human Rho guanine nucleotide exchange factor 8 ELISA Kit;Human ARHGEF8 ELISA Kit;Human NET1A ELISA Kit;Human neuroepithelial cell transforming 1 ELISA Kit;Human neuroepithelial cell-transforming gene 1 protein ELISA Kit;Human Rho guanine nucleotide exchange factor (GEF) 8 ELISA Kit;Human guanine nucleotide regulatory protein (oncogene) ELISA Kit;Human neuroepithelioma transforming gene 1 ELISA Kit;Human p65 Net1 proto-oncogene protein ELISA Kit;Human small GTP-binding protein regulator ELISA Kit;
  • Catalog
    E026140
  • species
    Human
  • GeneNET1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuroepithelial Cell Transforming Gene 1 (NET1). No significant cross-reactivity or interference between Neuroepithelial Cell Transforming Gene 1 (NET1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.125ng/mL.
  • Intended UseHuman NET1 ELISA Kit allows for the in vitro quantitative determination of NET1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroepithelial Cell Transforming Gene 1 (NET1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroepithelial Cell Transforming Gene 1 (NET1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroepithelial Cell Transforming Gene 1 (NET1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neuroepithelial cell-transforming gene 1 protein Protein information
  • Uniprot ID ARHG8_HUMAN
  • Uniprot AC Q7Z628; Q12773; Q96D82; Q99903; Q9UEN6;
  • UniGene Hs.25155;
  • GeneID 10276
  • KEGG hsa:10276;
  • Human Neuroepithelial cell-transforming gene 1 protein Protein SEQUENCE

  • SEQUENCE 596 AA; 67740 MW; E1AD964F14650D2C CRC64;

    MEPELAAQKQ PRPRRRSRRA SGLSTEGATG PSADTSGSEL DGRCSLRRGS

    SFTFLTPGPN WDFTLKRKRR EKDDDVVSLS SLDLKEPSNK RVRPLARVTS

    LANLISPVRN GAVRRFGQTI QSFTLRGDHR SPASAQKFSS RSTVPTPAKR

    RSSALWSEML DITMKESLTT REIRRQEAIY EMSRGEQDLI EDLKLARKAY

    HDPMLKLSIM SEEELTHIFG DLDSYIPLHE DLLTRIGEAT KPDGTVEQIG

    HILVSWLPRL NAYRGYCSNQ LAAKALLDQK KQDPRVQDFL QRCLESPFSR

    KLDLWSFLDI PRSRLVKYPL LLKEILKHTP KEHPDVQLLE DAILIIQGVL

    SDINLKKGES ECQYYIDKLE YLDEKQRDPR IEASKVLLCH GELRSKSGHK

    LYIFLFQDIL VLTRPVTRNE RHSYQVYRQP IPVQELVLED LQDGDVRMGG

    SFRGAFSNSE KAKNIFRIRF HDPSPAQSHT LQANDVFHKQ QWFNCIRAAI

    APFQSAGSPP ELQGLPELHE ECEGNHPSAR KLTAQRRAST VSSVTQVEVD

    ENAYRCGSGM QMAEDSKSLK THQTQPGIRR ARDKALSGGK RKETLV

  • UCSC uc001iia.5; human. [Q7Z628-1];



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