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Human NFASC ELISA Kit

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  • Alternative name

    Human KIAA0756 ELISA Kit;Human NF ELISA Kit;Human NRCAML ELISA Kit;Human neurofascin ELISA Kit;Human neurofascin homolog ELISA Kit;
  • Catalog
    E026142
  • species
    Human
  • GeneNFASC
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neurofascin (NFASC). No significant cross-reactivity or interference between Neurofascin (NFASC) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.115ng/mL.
  • Intended UseHuman NFASC ELISA Kit allows for the in vitro quantitative determination of NFASC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofascin (NFASC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofascin (NFASC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofascin (NFASC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofascin (NFASC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neurofascin Protein information
  • Uniprot ID NFASC_HUMAN
  • Uniprot AC Q5T2G0; Q5W9F8; Q68DH3; Q6ZQV6; Q7Z3K1; Q96HT1; Q96K50;
  • UniGene Hs.13349;
  • GeneID 23114
  • KEGG hsa:23114;
  • Human Neurofascin Protein SEQUENCE

  • SEQUENCE 1347 AA; 150027 MW; 4DC555E5AA06C223 CRC64;

    MARQPPPPWV HAAFLLCLLS LGGAIEIPMD PSIQNELTQP PTITKQSAKD

    HIVDPRDNIL IECEAKGNPA PSFHWTRNSR FFNIAKDPRV SMRRRSGTLV

    IDFRSGGRPE EYEGEYQCFA RNKFGTALSN RIRLQVSKSP LWPKENLDPV

    VVQEGAPLTL QCNPPPGLPS PVIFWMSSSM EPITQDKRVS QGHNGDLYFS

    NVMLQDMQTD YSCNARFHFT HTIQQKNPFT LKVLTTRGVA ERTPSFMYPQ

    GTASSQMVLR GMDLLLECIA SGVPTPDIAW YKKGGDLPSD KAKFENFNKA

    LRITNVSEED SGEYFCLASN KMGSIRHTIS VRVKAAPYWL DEPKNLILAP

    GEDGRLVCRA NGNPKPTVQW MVNGEPLQSA PPNPNREVAG DTIIFRDTQI

    SSRAVYQCNT SNEHGYLLAN AFVSVLDVPP RMLSPRNQLI RVILYNRTRL

    DCPFFGSPIP TLRWFKNGQG SNLDGGNYHV YENGSLEIKM IRKEDQGIYT

    CVATNILGKA ENQVRLEVKD PTRIYRMPED QVARRGTTVQ LECRVKHDPS

    LKLTVSWLKD DEPLYIGNRM KKEDDSLTIF GVAERDQGSY TCVASTELDQ

    DLAKAYLTVL ADQATPTNRL AALPKGRPDR PRDLELTDLA ERSVRLTWIP

    GDANNSPITD YVVQFEEDQF QPGVWHDHSK YPGSVNSAVL RLSPYVNYQF

    RVIAINEVGS SHPSLPSERY RTSGAPPESN PGDVKGEGTR KNNMEITWTP

    MNATSAFGPN LRYIVKWRRR ETREAWNNVT VWGSRYVVGQ TPVYVPYEIR

    VQAENDFGKG PEPESVIGYS GEDYPRAAPT EVKVRVMNST AISLQWNRVY

    SDTVQGQLRE YRAYYWRESS LLKNLWVSQK RQQASFPGDR LRGVVSRLFP

    YSNYKLEMVV VNGRGDGPRS ETKEFTTPEG VPSAPRRFRV RQPNLETINL

    EWDHPEHPNG IMIGYTLKYV AFNGTKVGKQ IVENFSPNQT KFTVQRTDPV

    SRYRFTLSAR TQVGSGEAVT EESPAPPNEA TPTAAPPTLP PTTVGATGAV

    SSTDATAIAA TTEATTVPII PTVAPTTIAT TTTVATTTTT TAAATTTTES

    PPTTTSGTKI HESAPDEQSI WNVTVLPNSK WANITWKHNF GPGTDFVVEY

    IDSNHTKKTV PVKAQAQPIQ LTDLYPGMTY TLRVYSRDNE GISSTVITFM

    TSTAYTNNQA DIATQGWFIG LMCAIALLVL ILLIVCFIKR SRGGKYPVRE

    KKDVPLGPED PKEEDGSFDY SDEDNKPLQG SQTSLDGTIK QQESDDSLVD

    YGEGGEGQFN EDGSFIGQYT VKKDKEETEG NESSEATSPV NAIYSLA

  • UCSC uc001hbh.4; human. [O94856-1];



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