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Human NEFL ELISA Kit

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  • Alternative name

    Human NF-L ELISA Kit;Human 68 kDa neurofilament protein ELISA Kit;Human neurofilament triplet L protein ELISA Kit;Human NF68 ELISA Kit;Human NFL ELISA Kit;Human CMT1F ELISA Kit;Human CMT2E ELISA Kit;Human PPP1R110 ELISA Kit;Human neurofilament light ELISA Kit;Human neurofilament light polypeptide ELISA Kit;Human light molecular weight neurofilament protein ELISA Kit;Human neurofilament protein, light chain ELISA Kit;Human neurofilament subunit NF-L ELISA Kit;Human neurofilament, light polypeptide 68kDa ELISA Kit;Human protein phosphatase 1, regulatory subunit 110 ELISA Kit;
  • Catalog
    E026155
  • species
    Human
  • GeneNEFL
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NEFL . No significant cross-reactivity or interference between NEFL and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.375pg/ml
  • Intended UseHuman NEFL ELISA Kit allows for the in vitro quantitative determination of NEFL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NEFL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NEFL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NEFL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NEFL can be calculated.
  • Human Neurofilament light polypeptide Protein information
  • Uniprot ID NFL_HUMAN
  • Uniprot AC P07196; B9ZVN2; Q16154; Q8IU72;
  • UniGene Hs.521461;
  • GeneID 4747
  • KEGG hsa:4747;
  • Human Neurofilament light polypeptide Protein SEQUENCE

  • SEQUENCE 543 AA; 61517 MW; 98C1712D8ACFF33A CRC64;

    MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS

    LSVRRSYSSS SGSLMPSLEN LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR

    FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA

    EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD

    EAALARAELE KRIDSLMDEI SFLKKVHEEE IAELQAQIQY AQISVEMDVT

    KPDLSAALKD IRAQYEKLAA KNMQNAEEWF KSRFTVLTES AAKNTDAVRA

    AKDEVSESRR LLKAKTLEIE ACRGMNEALE KQLQELEDKQ NADISAMQDT

    INKLENELRT TKSEMARYLK EYQDLLNVKM ALDIEIAAYR KLLEGEETRL

    SFTSVGSITS GYSQSSQVFG RSAYGGLQTS SYLMSTRSFP SYYTSHVQEE

    QIEVEETIEA AKAEEAKDEP PSEGEAEEEE KDKEEAEEEE AAEEEEAAKE

    ESEEAKEEEE GGEGEEGEET KEAEEEEKKV EGAGEEQAAK KKD

  • UCSC uc033bfe.2; human.;



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