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Human NEFH ELISA Kit

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  • Alternative name

    Human NF-H ELISA Kit;Human 200 kDa neurofilament protein ELISA Kit;Human neurofilament triplet H protein ELISA Kit;Human KIAA0845 ELISA Kit;Human NFH ELISA Kit;Human CMT2CC ELISA Kit;Human neurofilament heavy ELISA Kit;Human neurofilament heavy polypeptide ELISA Kit;Human neurofilament, heavy polypeptide 200kDa ELISA Kit;
  • Catalog
    E026161
  • species
    Human
  • GeneNEFH
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NEFH. No significant cross-reactivity or interference between Human NEFH and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.156 ng/ml
  • Intended UseHuman NEFH ELISA Kit allows for the in vitro quantitative determination of NEFH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-H. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NF-H and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain NF-H, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NF-H in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Neurofilament heavy polypeptide Protein information
  • Uniprot ID NFH_HUMAN
  • Uniprot AC P12036; B4DYY4; Q96HF8; Q9UJS7; Q9UQ14;
  • UniGene Hs.198760;
  • GeneID 4744
  • KEGG hsa:4744;
  • Human Neurofilament heavy polypeptide Protein SEQUENCE

  • SEQUENCE 1026 AA; 112479 MW; 0879B6A08D208C17 CRC64;

    MMSFGGADAL LGAPFAPLHG GGSLHYALAR KGGAGGTRSA AGSSSGFHSW

    TRTSVSSVSA SPSRFRGAGA ASSTDSLDTL SNGPEGCMVA VATSRSEKEQ

    LQALNDRFAG YIDKVRQLEA HNRSLEGEAA ALRQQQAGRS AMGELYEREV

    REMRGAVLRL GAARGQLRLE QEHLLEDIAH VRQRLDDEAR QREEAEAAAR

    ALARFAQEAE AARVDLQKKA QALQEECGYL RRHHQEEVGE LLGQIQGSGA

    AQAQMQAETR DALKCDVTSA LREIRAQLEG HAVQSTLQSE EWFRVRLDRL

    SEAAKVNTDA MRSAQEEITE YRRQLQARTT ELEALKSTKD SLERQRSELE

    DRHQADIASY QEAIQQLDAE LRNTKWEMAA QLREYQDLLN VKMALDIEIA

    AYRKLLEGEE CRIGFGPIPF SLPEGLPKIP SVSTHIKVKS EEKIKVVEKS

    EKETVIVEEQ TEETQVTEEV TEEEEKEAKE EEGKEEEGGE EEEAEGGEEE

    TKSPPAEEAA SPEKEAKSPV KEEAKSPAEA KSPEKEEAKS PAEVKSPEKA

    KSPAKEEAKS PPEAKSPEKE EAKSPAEVKS PEKAKSPAKE EAKSPAEAKS

    PEKAKSPVKE EAKSPAEAKS PVKEEAKSPA EVKSPEKAKS PTKEEAKSPE

    KAKSPEKAKS PEKEEAKSPE KAKSPVKAEA KSPEKAKSPV KAEAKSPEKA

    KSPVKEEAKS PEKAKSPVKE EAKSPEKAKS PVKEEAKTPE KAKSPVKEEA

    KSPEKAKSPE KAKTLDVKSP EAKTPAKEEA RSPADKFPEK AKSPVKEEVK

    SPEKAKSPLK EDAKAPEKEI PKKEEVKSPV KEEEKPQEVK VKEPPKKAEE

    EKAPATPKTE EKKDSKKEEA PKKEAPKPKV EEKKEPAVEK PKESKVEAKK

    EEAEDKKKVP TPEKEAPAKV EVKEDAKPKE KTEVAKKEPD DAKAKEPSKP

    AEKKEAAPEK KDTKEEKAKK PEEKPKTEAK AKEDDKTLSK EPSKPKAEKA

    EKSSSTDQKD SKPPEKATED KAAKGK

  • UCSC uc003afo.4; human. [P12036-1];



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