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Human NPFF ELISA Kit

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  • Alternative name

    Human FMRFamide-related peptides ELISA Kit;Human NPSF ELISA Kit;Human NPFF ELISA Kit;Human NPAF ELISA Kit;Human FMRFAL ELISA Kit;Human neuropeptide FF-amide peptide precursor ELISA Kit;Human pro-FMRFamide-related neuropeptide FF ELISA Kit;
  • Catalog
    E026296
  • species
    Human
  • GeneNPFF
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF). No significant cross-reactivity or interference between Neuropeptide FF (NPFF) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 4.54pg/mL.
  • Intended UseHuman NPFF ELISA Kit allows for the in vitro quantitative determination of NPFF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide FF (NPFF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide FF (NPFF) and unlabeled Neuropeptide FF (NPFF) (Standards or samples) with the pre-coated antibody specific to Neuropeptide FF (NPFF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample.
  • Human Neuropeptide AF Protein information
  • Uniprot ID NPFF_HUMAN
  • Uniprot AC O15130; Q3SXL4;
  • UniGene Hs.733076;
  • GeneID 8620
  • KEGG hsa:8620;
  • Human Neuropeptide AF Protein SEQUENCE

  • SEQUENCE 113 AA; 12440 MW; 1E9D4ED2A69238E3 CRC64;

    MDSRQAAALL VLLLLIDGGC AEGPGGQQED QLSAEEDSEP LPPQDAQTSG

    SLLHYLLQAM ERPGRSQAFL FQPQRFGRNT QGSWRNEWLS PRAGEGLNSQ

    FWSLAAPQRF GKK

  • UCSC uc001sdw.1; human. [O15130-1];



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