Human ELANE ELISA Kit

Alternative name
Human bone marrow serine protease ELISA Kit;Human elastase-2 ELISA Kit;Human Human leukocyte elastase ELISA Kit;Human HLE ELISA Kit;Human medullasin ELISA Kit;Human PMN elastase ELISA Kit;Human ELA2 ELISA Kit;Human GE ELISA Kit;Human HNE ELISA Kit;Human NE ELISA Kit;Human PMN-E ELISA Kit;Human SCN1 ELISA Kit;Human elastase, neutrophil expressed ELISA Kit;Human neutrophil elastase ELISA Kit;Human elastase 2, neutrophil ELISA Kit;Human granulocyte-derived elastase ELISA Kit;Human leukocyte elastase ELISA Kit;Human polymorphonuclear elastase ELISA Kit;
Catalog
E026429
species
Human
GeneELANE
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman ELANE ELISA Kit allows for the in vitro quantitative determination of ELANE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human NEantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NE antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman NEcan be calculated.
Human Neutrophil elastase Protein information
Uniprot ID ELNE_HUMAN
Uniprot AC P08246; P09649; Q6B0D9; Q6LDP5;
UniGene Hs.99863;
GeneID 1991
KEGG hsa:1991;
Human Neutrophil elastase Protein SEQUENCE
SEQUENCE 267 AA; 28518 MW; 3F7610DC33CAA4B9 CRC64;
MTLGRRLACL FLACVLPALL LGGTALASEI VGGRRARPHA WPFMVSLQLR
GGHFCGATLI APNFVMSAAH CVANVNVRAV RVVLGAHNLS RREPTRQVFA
VQRIFENGYD PVNLLNDIVI LQLNGSATIN ANVQVAQLPA QGRRLGNGVQ
CLAMGWGLLG RNRGIASVLQ ELNVTVVTSL CRRSNVCTLV RGRQAGVCFG
DSGSPLVCNG LIHGIASFVR GGCASGLYPD AFAPVAQFVN WIDSIIQRSE
DNPCPHPRDP DPASRTH
UCSC uc002lqb.4; human.;

Category

Human ELANE ELISA Kit