Human NFKBIE ELISA Kit

Alternative name
Human NF-kappa-BIE ELISA Kit;Human I-kappa-B-epsilon ELISA Kit;Human ikB-E ELISA Kit;Human ikB-epsilon ELISA Kit;Human ikappaBepsilon ELISA Kit;Human IKBE ELISA Kit;Human NFKB inhibitor epsilon ELISA Kit;Human NF-kappa-B inhibitor epsilon ELISA Kit;Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon ELISA Kit;
Catalog
E026482
species
Human
GeneNFKBIE
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human Nfkbie. No significant cross-reactivity or interference between Human Nfkbie and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human Nfkbie and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman NFKBIE ELISA Kit allows for the in vitro quantitative determination of NFKBIE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Nfkbie in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Nfkbie. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human Nfkbie and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Nfkbie, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Nfkbie. You can calculate the concentration of Human Nfkbie in the samples by comparing the OD of the samples to the standard curve.
Human NF-kappa-B inhibitor epsilon Protein information
Uniprot ID IKBE_HUMAN
Uniprot AC O00221; Q5T9V9;
UniGene Hs.458276;
GeneID 4794
KEGG hsa:4794;
Human NF-kappa-B inhibitor epsilon Protein SEQUENCE
SEQUENCE 500 AA; 52864 MW; 45E726E7A0E8D478 CRC64;
MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR
PCWSGGAGRG GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR
PPRAGRPLCQ ADPQPGKAAR RSLEPDPAQT GPRPARAAGM SEARKGPDEA
EESQYDSGIE SLRSLRSLPE STSAPASGPS DGSPQPCTHP PGPVKEPQEK
EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE
ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL
AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR
PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE
GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP LHLAAGRGLM
GISSTLCKAG ADSLLRNVED ETPQDLTEES LVLLPFDDLK ISGKLLLCTD
UCSC uc003oxe.1; human.;

Category

Human NFKBIE ELISA Kit