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Human NOS1 ELISA Kit

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  • Alternative name

    Human constitutive NOS ELISA Kit;Human NC-NOS ELISA Kit;Human NOS type I ELISA Kit;Human neuronal NOS ELISA Kit;Human N-NOS ELISA Kit;Human nNOS ELISA Kit;Human peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit;Human bNOS ELISA Kit;Human IHPS1 ELISA Kit;Human NOS ELISA Kit;Human nitric oxide synthase 1 ELISA Kit;Human nitric oxide synthase, brain ELISA Kit;Human nitric oxide synthase 1 (neuronal) ELISA Kit;
  • Catalog
    E026616
  • species
    Human
  • GeneNOS1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NOS1. No significant cross-reactivity or interference between Human NOS1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.039 IU/ml
  • Intended UseHuman NOS1 ELISA Kit allows for the in vitro quantitative determination of NOS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to nNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for nNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain nNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of nNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nitric oxide synthase, brain Protein information
  • Uniprot ID NOS1_HUMAN
  • Uniprot AC P29475; E9PH30; O75713;
  • UniGene Hs.654410; Hs.684465; Hs.684466; Hs.684467; Hs.735734;
  • GeneID 4842
  • KEGG hsa:4842;
  • Human Nitric oxide synthase, brain Protein SEQUENCE

  • SEQUENCE 1434 AA; 160970 MW; 99235793B953BF37 CRC64;

    MEDHMFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG

    AAEQSGLIQA GDIILAVNGR PLVDLSYDSA LEVLRGIASE THVVLILRGP

    EGFTTHLETT FTGDGTPKTI RVTQPLGPPT KAVDLSHQPP AGKEQPLAVD

    GASGPGNGPQ HAYDDGQEAG SLPHANGLAP RPPGQDPAKK ATRVSLQGRG

    ENNELLKEIE PVLSLLTSGS RGVKGGAPAK AEMKDMGIQV DRDLDGKSHK

    PLPLGVENDR VFNDLWGKGN VPVVLNNPYS EKEQPPTSGK QSPTKNGSPS

    KCPRFLKVKN WETEVVLTDT LHLKSTLETG CTEYICMGSI MHPSQHARRP

    EDVRTKGQLF PLAKEFIDQY YSSIKRFGSK AHMERLEEVN KEIDTTSTYQ

    LKDTELIYGA KHAWRNASRC VGRIQWSKLQ VFDARDCTTA HGMFNYICNH

    VKYATNKGNL RSAITIFPQR TDGKHDFRVW NSQLIRYAGY KQPDGSTLGD

    PANVQFTEIC IQQGWKPPRG RFDVLPLLLQ ANGNDPELFQ IPPELVLEVP

    IRHPKFEWFK DLGLKWYGLP AVSNMLLEIG GLEFSACPFS GWYMGTEIGV

    RDYCDNSRYN ILEEVAKKMN LDMRKTSSLW KDQALVEINI AVLYSFQSDK

    VTIVDHHSAT ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM

    LNYRLTPSFE YQPDPWNTHV WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM

    GQAMAKRVKA TILYATETGK SQAYAKTLCE IFKHAFDAKV MSMEEYDIVH

    LEHETLVLVV TSTFGNGDPP ENGEKFGCAL MEMRHPNSVQ EERKSYKVRF

    NSVSSYSDSQ KSSGDGPDLR DNFESAGPLA NVRFSVFGLG SRAYPHFCAF

    GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG

    DDVNIEKANN SLISNDRSWK RNKFRLTFVA EAPELTQGLS NVHKKRVSAA

    RLLSRQNLQS PKSSRSTIFV RLHTNGSQEL QYQPGDHLGV FPGNHEDLVN

    ALIERLEDAP PVNQMVKVEL LEERNTALGV ISNWTDELRL PPCTIFQAFK

    YYLDITTPPT PLQLQQFASL ATSEKEKQRL LVLSKGLQEY EEWKWGKNPT

    IVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPDMYP DEVHLTVAIV

    SYRTRDGEGP IHHGVCSSWL NRIQADELVP CFVRGAPSFH LPRNPQVPCI

    LVGPGTGIAP FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE

    TLQAKNKGVF RELYTAYSRE PDKPKKYVQD ILQEQLAESV YRALKEQGGH

    IYVCGDVTMA ADVLKAIQRI MTQQGKLSAE DAGVFISRMR DDNRYHEDIF

    GVTLRTYEVT NRLRSESIAF IEESKKDTDE VFSS

  • UCSC uc001twm.3; human. [P29475-1];



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