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Human NOS2 ELISA Kit

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  • Alternative name

    Human hepatocyte NOS ELISA Kit;Human HEP-NOS ELISA Kit;Human inducible NO synthase ELISA Kit;Human inducible NOS ELISA Kit;Human INOS ELISA Kit;Human NOS type II ELISA Kit;Human peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit;Human NOS2A ELISA Kit;Human NOS ELISA Kit;Human nitric oxide synthase 2 ELISA Kit;Human nitric oxide synthase, inducible ELISA Kit;Human NOS, type II ELISA Kit;Human nitric oxide synthase 2, inducible ELISA Kit;Human nitric oxide synthase 2A (inducible, hepatocytes) ELISA Kit;Human nitric oxide synthase, macrophage ELISA Kit;
  • Catalog
    E026619
  • species
    Human
  • GeneNOS2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NOS2. No significant cross-reactivity or interference between Human NOS2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.225 IU/ml
  • Intended UseHuman NOS2 ELISA Kit allows for the in vitro quantitative determination of NOS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to iNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for iNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain iNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of iNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Nitric oxide synthase, inducible Protein information
  • Uniprot ID NOS2_HUMAN
  • Uniprot AC Q9UD42;
  • UniGene Hs.709191;
  • GeneID 4843
  • KEGG hsa:4843;
  • Human Nitric oxide synthase, inducible Protein SEQUENCE

  • SEQUENCE 1153 AA; 131117 MW; 47671E5385CB3A52 CRC64;

    MACPWKFLFK TKFHQYAMNG EKDINNNVEK APCATSSPVT QDDLQYHNLS

    KQQNESPQPL VETGKKSPES LVKLDATPLS SPRHVRIKNW GSGMTFQDTL

    HHKAKGILTC RSKSCLGSIM TPKSLTRGPR DKPTPPDELL PQAIEFVNQY

    YGSFKEAKIE EHLARVEAVT KEIETTGTYQ LTGDELIFAT KQAWRNAPRC

    IGRIQWSNLQ VFDARSCSTA REMFEHICRH VRYSTNNGNI RSAITVFPQR

    SDGKHDFRVW NAQLIRYAGY QMPDGSIRGD PANVEFTQLC IDLGWKPKYG

    RFDVVPLVLQ ANGRDPELFE IPPDLVLEVA MEHPKYEWFR ELELKWYALP

    AVANMLLEVG GLEFPGCPFN GWYMGTEIGV RDFCDVQRYN ILEEVGRRMG

    LETHKLASLW KDQAVVEINI AVLHSFQKQN VTIMDHHSAA ESFMKYMQNE

    YRSRGGCPAD WIWLVPPMSG SITPVFHQEM LNYVLSPFYY YQVEAWKTHV

    WQDEKRRPKR REIPLKVLVK AVLFACMLMR KTMASRVRVT ILFATETGKS

    EALAWDLGAL FSCAFNPKVV CMDKYRLSCL EEERLLLVVT STFGNGDCPG

    NGEKLKKSLF MLKELNNKFR YAVFGLGSSM YPRFCAFAHD IDQKLSHLGA

    SQLTPMGEGD ELSGQEDAFR SWAVQTFKAA CETFDVRGKQ HIQIPKLYTS

    NVTWDPHHYR LVQDSQPLDL SKALSSMHAK NVFTMRLKSR QNLQSPTSSR

    ATILVELSCE DGQGLNYLPG EHLGVCPGNQ PALVQGILER VVDGPTPHQT

    VRLEALDESG SYWVSDKRLP PCSLSQALTY FLDITTPPTQ LLLQKLAQVA

    TEEPERQRLE ALCQPSEYSK WKFTNSPTFL EVLEEFPSLR VSAGFLLSQL

    PILKPRFYSI SSSRDHTPTE IHLTVAVVTY HTRDGQGPLH HGVCSTWLNS

    LKPQDPVPCF VRNASGFHLP EDPSHPCILI GPGTGIAPFR SFWQQRLHDS

    QHKGVRGGRM TLVFGCRRPD EDHIYQEEML EMAQKGVLHA VHTAYSRLPG

    KPKVYVQDIL RQQLASEVLR VLHKEPGHLY VCGDVRMARD VAHTLKQLVA

    AKLKLNEEQV EDYFFQLKSQ KRYHEDIFGA VFPYEAKKDR VAVQPSSLEM

    SAL

  • UCSC uc002gzu.4; human. [P35228-1];



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