Human NOTUM ELISA Kit

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  • Alternative name

    Human hNOTUM ELISA Kit;Human NOTUM, palmitoleoyl-protein carboxylesterase ELISA Kit;Human palmitoleoyl-protein carboxylesterase NOTUM ELISA Kit;Human [Wnt protein] O-palmitoleoyl-L-serine hydrolase ELISA Kit;Human notum pectinacetylesterase homolog ELISA Kit;Human protein notum homolog ELISA Kit;

  • Catalog
    E026807
  • species
    Human
  • GeneNOTUM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human NOTUM. No significant cross-reactivity or interference between human NOTUM and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human NOTUM is typically less than 0.39ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman NOTUM ELISA Kit allows for the in vitro quantitative determination of NOTUM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOTUM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOTUM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOTUM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOTUM bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Palmitoleoyl-protein carboxylesterase NOTUM Protein information
  • Uniprot ID NOTUM_HUMAN
  • Uniprot AC Q6P988; Q8N410; Q8NI82;
  • UniGene Hs.106137;
  • GeneID 147111
  • KEGG hsa:147111;
  • Human Palmitoleoyl-protein carboxylesterase NOTUM Protein SEQUENCE
  • SEQUENCE 496 AA; 55699 MW; 00A7B3FB6436F544 CRC64;

    MGRGVRVLLL LSLLHCAGGS EGRKTWRRRG QQPPPPPRTE AAPAAGQPVE

    SFPLDFTAVE GNMDSFMAQV KSLAQSLYPC SAQQLNEDLR LHLLLNTSVT

    CNDGSPAGYY LKESRGSRRW LLFLEGGWYC FNRENCDSRY DTMRRLMSSR

    DWPRTRTGTG ILSSQPEENP YWWNANMVFI PYCSSDVWSG ASSKSEKNEY

    AFMGALIIQE VVRELLGRGL SGAKVLLLAG SSAGGTGVLL NVDRVAEQLE

    KLGYPAIQVR GLADSGWFLD NKQYRHTDCV DTITCAPTEA IRRGIRYWNG

    VVPERCRRQF QEGEEWNCFF GYKVYPTLRC PVFVVQWLFD EAQLTVDNVH

    LTGQPVQEGL RLYIQNLGRE LRHTLKDVPA SFAPACLSHE IIIRSHWTDV

    QVKGTSLPRA LHCWDRSLHD SHKASKTPLK GCPVHLVDSC PWPHCNPSCP

    TVRDQFTGQE MNVAQFLMHM GFDMQTVAQP QGLEPSELLG MLSNGS

  • UCSC uc010wvg.3; human.;



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