Human NFIB ELISA Kit

Alternative name
Human NF1-B ELISA Kit;Human nuclear factor 1/B ELISA Kit;Human CCAAT-box-binding transcription factor ELISA Kit;Human CTF ELISA Kit;Human Nuclear factor I/B ELISA Kit;Human NF-I/B ELISA Kit;Human NFI-B ELISA Kit;Human TGGCA-binding protein ELISA Kit;Human HMGIC/NFIB ELISA Kit;Human NFI-RED ELISA Kit;Human NFIB2 ELISA Kit;Human NFIB3 ELISA Kit;Human nuclear factor I B ELISA Kit;Human nuclear factor 1 B-type ELISA Kit;
Catalog
E026883
species
Human
GeneNFIB
SpecificityThis assay has high sensitivity and excellent specificity for detection of NFIB. No significant cross-reactivity or interference between NFIB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human NFIB is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman NFIB ELISA Kit allows for the in vitro quantitative determination of NFIB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFIB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NFIB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFIB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NFIB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Nuclear factor 1 B-type Protein information
Uniprot ID NFIB_HUMAN
Uniprot AC Q96J45;
UniGene Hs.644095;
GeneID 4781
KEGG hsa:4781;
Human Nuclear factor 1 B-type Protein SEQUENCE
SEQUENCE 420 AA; 47442 MW; 0F71635FE9D64FA4 CRC64;
MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS
KDEERAVKDE LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK
HPCCVLSNPD QKGKIRRIDC LRQADKVWRL DLVMVILFKG IPLESTDGER
LMKSPHCTNP ALCVQPHHIT VSVKELDLFL AYYVQEQDSG QSGSPSHNDP
AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP IGEIPSQPYY
HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS
RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV
AHSVISTRTP PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK
NYVPSYDPSS PQTSQSWYLG
UCSC uc003zld.4; human. [O00712-1];

Category

Human NFIB ELISA Kit