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Human NUP153 ELISA Kit

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  • Alternative name

    Human 153 kDa nucleoporin ELISA Kit;Human nucleoporin Nup153 ELISA Kit;Human HNUP153 ELISA Kit;Human N153 ELISA Kit;Human nucleoporin 153 ELISA Kit;Human nuclear pore complex protein Nup153 ELISA Kit;Human nuclear pore complex protein hnup153 ELISA Kit;Human nucleoporin 153kD ELISA Kit;Human nucleoporin 153kDa ELISA Kit;
  • Catalog
    E027088
  • species
    Human
  • GeneNUP153
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of NUP153. No significant cross-reactivity or interference between NUP153 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NUP153 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman NUP153 ELISA Kit allows for the in vitro quantitative determination of NUP153 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses: This NUP153 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NUP153. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||NUP153 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NUP153 antibody and an NUP153-HRP conjugate. The assay sample and buffer are incubated together with NUP153-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NUP153 concentration since NUP153 from samples and NUP153-HRP conjugate compete for the anti-NUP153 antibody binding site. Since the number of sites is limited, as more sites are occupied by NUP153 from the sample, fewer sites are left to bind NUP153-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NUP153 concentration in each sample is interpolated from this standard curve.
  • Human Nuclear pore complex protein Nup153 Protein information
  • Uniprot ID NU153_HUMAN
  • Uniprot AC P49790; B4DIK2; E7EPX5; F6QR24; Q4LE47; Q5T9I7; Q7Z743;
  • UniGene Hs.601591;
  • GeneID 9972
  • KEGG hsa:9972;
  • Human Nuclear pore complex protein Nup153 Protein SEQUENCE

  • SEQUENCE 1475 AA; 153938 MW; D07455A691F7CD1F CRC64;

    MASGAGGVGG GGGGKIRTRR CHQGPIKPYQ QGRQQHQGIL SRVTESVKNI

    VPGWLQRYFN KNEDVCSCST DTSEVPRWPE NKEDHLVYAD EESSNITDGR

    ITPEPAVSNT EEPSTTSTAS NYPDVLTRPS LHRSHLNFSM LESPALHCQP

    STSSAFPIGS SGFSLVKEIK DSTSQHDDDN ISTTSGFSSR ASDKDITVSK

    NTSLPPLWSP EAERSHSLSQ HTATSSKKPA FNLSAFGTLS PSLGNSSILK

    TSQLGDSPFY PGKTTYGGAA AAVRQSKLRN TPYQAPVRRQ MKAKQLSAQS

    YGVTSSTARR ILQSLEKMSS PLADAKRIPS IVSSPLNSPL DRSGIDITDF

    QAKREKVDSQ YPPVQRLMTP KPVSIATNRS VYFKPSLTPS GEFRKTNQRI

    DNKCSTGYEK NMTPGQNREQ RESGFSYPNF SLPAANGLSS GVGGGGGKMR

    RERTRFVASK PLEEEEMEVP VLPKISLPIT SSSLPTFNFS SPEITTSSPS

    PINSSQALTN KVQMTSPSST GSPMFKFSSP IVKSTEANVL PPSSIGFTFS

    VPVAKTAELS GSSSTLEPII SSSAHHVTTV NSTNCKKTPP EDCEGPFRPA

    EILKEGSVLD ILKSPGFASP KIDSVAAQPT ATSPVVYTRP AISSFSSSGI

    GFGESLKAGS SWQCDTCLLQ NKVTDNKCIA CQAAKLSPRD TAKQTGIETP

    NKSGKTTLSA SGTGFGDKFK PVIGTWDCDT CLVQNKPEAI KCVACETPKP

    GTCVKRALTL TVVSESAETM TASSSSCTVT TGTLGFGDKF KRPIGSWECS

    VCCVSNNAED NKCVSCMSEK PGSSVPASSS STVPVSLPSG GSLGLEKFKK

    PEGSWDCELC LVQNKADSTK CLACESAKPG TKSGFKGFDT SSSSSNSAAS

    SSFKFGVSSS SSGPSQTLTS TGNFKFGDQG GFKIGVSSDS GSINPMSEGF

    KFSKPIGDFK FGVSSESKPE EVKKDSKNDN FKFGLSSGLS NPVSLTPFQF

    GVSNLGQEEK KEELPKSSSA GFSFGTGVIN STPAPANTIV TSENKSSFNL

    GTIETKSASV APFTCKTSEA KKEEMPATKG GFSFGNVEPA SLPSASVFVL

    GRTEEKQQEP VTSTSLVFGK KADNEEPKCQ PVFSFGNSEQ TKDENSSKST

    FSFSMTKPSE KESEQPAKAT FAFGAQTSTT ADQGAAKPVF SFLNNSSSSS

    STPATSAGGG IFGSSTSSSN PPVATFVFGQ SSNPVSSSAF GNTAESSTSQ

    SLLFSQDSKL ATTSSTGTAV TPFVFGPGAS SNNTTTSGFG FGATTTSSSA

    GSSFVFGTGP SAPSASPAFG ANQTPTFGQS QGASQPNPPG FGSISSSTAL

    FPTGSQPAPP TFGTVSSSSQ PPVFGQQPSQ SAFGSGTTPN SSSAFQFGSS

    TTNFNFTNNS PSGVFTFGAN SSTPAASAQP SGSGGFPFNQ SPAAFTVGSN

    GKNVFSSSGT SFSGRKIKTA VRRRK

  • UCSC uc003ncd.3; human. [P49790-1];



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