Human OCLN ELISA Kit

Alternative name
Human BLCPMG ELISA Kit;Human PPP1R115 ELISA Kit;Human occludin ELISA Kit;Human phosphatase 1, regulatory subunit 115 ELISA Kit;Human tight junction protein occludin ELISA Kit;
Catalog
E027218
species
Human
GeneOCLN
SpecificityThis assay has high sensitivity and excellent specificity for detection of human OCLN. No significant cross-reactivity or interference between human OCLN and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human OCLN is typically less than 5.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseHuman OCLN ELISA Kit allows for the in vitro quantitative determination of OCLN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OCLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OCLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OCLN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OCLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Occludin Protein information
Uniprot ID OCLN_HUMAN
Uniprot AC Q8N6K1;
UniGene Hs.592605;
GeneID 100506658
KEGG hsa:100506658;
Human Occludin Protein SEQUENCE
SEQUENCE 522 AA; 59144 MW; A0CF9574BCF6E974 CRC64;
MSSRPLESPP PYRPDEFKPN HYAPSNDIYG GEMHVRPMLS QPAYSFYPED
EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTSLLGG
SVGYPYGGSG FGSYGSGYGY GYGYGYGYGG YTDPRAAKGF MLAMAAFCFI
AALVIFVTSV IRSEMSRTRR YYLSVIIVSA ILGIMVFIAT IVYIMGVNPT
AQSSGSLYGS QIYALCNQFY TPAATGLYVD QYLYHYCVVD PQEAIAIVLG
FMIIVAFALI IFFAVKTRRK MDRYDKSNIL WDKEHIYDEQ PPNVEEWVKN
VSAGTQDVPS PPSDYVERVD SPMAYSSNGK VNDKRFYPES SYKSTPVPEV
VQELPLTSPV DDFRQPRYSS GGNFETPSKR APAKGRAGRS KRTEQDHYET
DYTTGGESCD ELEEDWIREY PPITSDQQRQ LYKRNFDTGL QEYKSLQSEL
DEINKELSRL DKELDDYREE SEEYMAAADE YNRLKQVKGS ADYKSKKNHC
KQLKSKLSHI KKMVGDYDRQ KT
UCSC uc003jwu.3; human. [Q16625-1];

Category

Human OCLN ELISA Kit

Human BLCPMG ELISA Kit;Human PPP1R115 ELISA Kit;Human occludin ELISA Kit;Human phosphatase 1, regulatory subunit 115 ELISA Kit;Human tight junction protein occludin ELISA Kit;