Human OR14I1 ELISA Kit

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  • Alternative name

    Human olfactory receptor 5BU1 ELISA Kit;Human OR5BU1 ELISA Kit;Human OR5BU1P ELISA Kit;Human olfactory receptor family 14 subfamily I member 1 ELISA Kit;Human olfactory receptor 14I1 ELISA Kit;Human olfactory receptor, family 5, subfamily BU, member 1 pseudogene ELISA Kit;

  • Catalog
    E027342
  • species
    Human
  • GeneOR14I1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of OR14I1. No significant cross-reactivity or interference between OR14I1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between OR14I1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman OR14I1 ELISA Kit allows for the in vitro quantitative determination of OR14I1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This OR14I1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OR14I1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||OR14I1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-OR14I1 antibody and an OR14I1-HRP conjugate. The assay sample and buffer are incubated together with OR14I1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OR14I1 concentration since OR14I1 from samples and OR14I1-HRP conjugate compete for the anti-OR14I1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OR14I1 from the sample, fewer sites are left to bind OR14I1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OR14I1 concentration in each sample is interpolated from this standard curve.
  • Human Olfactory receptor 14I1 Protein information
  • Uniprot ID O14I1_HUMAN
  • Uniprot AC A6ND48;
  • UniGene Hs.449973;
  • GeneID 401994
  • KEGG hsa:401994;
  • Human Olfactory receptor 14I1 Protein SEQUENCE
  • SEQUENCE 311 AA; 35175 MW; 2AC007546D7A9C59 CRC64;

    MDNLTKVTEF LLMEFSGIWE LQVLHAGLFL LIYLAVLVGN LLIIAVITLD

    QHLHTPMYFF LKNLSVLDLC YISVTVPKSI RNSLTRRSSI SYLGCVAQVY

    FFSAFASAEL AFLTVMSYDR YVAICHPLQY RAVMTSGGCY QMAVTTWLSC

    FSYAAVHTGN MFREHVCRSS VIHQFFRDIP HVLALVSCEV FFVEFLTLAL

    SSCLVLGCFI LMMISYFQIF STVLRIPSGQ SRAKAFSTCS PQLIVIMLFL

    TTGLFAALGP IAKALSIQDL VIALTYTVLP PFLNPIIYSL RNKEIKTAMW

    RLFVKIYFLQ K

  • UCSC uc001ieu.1; human.;



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