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Alternative name
Human OM ELISA Kit;Human parvalbumin beta ELISA Kit;Human OCM1 ELISA Kit;Human OCMN ELISA Kit;Human ONCM ELISA Kit;Human oncomodulin ELISA Kit;Human oncomodulin-1 ELISA Kit;Human beta parvalbumin ELISA Kit;Human hCG18255 ELISA Kit;
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Catalog
E027677
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species
Human
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GeneOCM
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SpecificityNatural and recombinant Human Oncomodulin
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity Intra-assay Precision Intra-Assay Precision (Precision within an assay) Three samples of kn
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Intended UseHuman OCM ELISA Kit allows for the in vitro quantitative determination of OCM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay: Human Oncomodulin ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Oncomodulin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli; Immunogen sequence: S2-S109) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Oncomodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Oncomodulin amount of sample captured in plate.
Background/Introduction: Oncomodulin, also known as OCM, is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. The human gene for oncomodulin is located on chromosome 7. It is a parvalbumin- family calcium-binding protein expressed and secreted by macrophages that typically traffic to tissue as an inflammatory response or after injury. Oncomodulin promotes axon regeneration in retinal ganglion cells and maintains functioning in mouse cochlear hair cells.
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Human Oncomodulin-1 Protein information
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Uniprot ID
ONCO_HUMAN
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Uniprot AC
P0CE72; B9EJH7; P32930; Q6ISI5; Q75MW0;
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UniGene
Hs.571315;
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GeneID
654231
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KEGG
hsa:654231;
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Human Oncomodulin-1 Protein SEQUENCE
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SEQUENCE 109 AA; 12184 MW; C5793E2FF4A058A0 CRC64;
MSITDVLSAD DIAAALQECR DPDTFEPQKF FQTSGLSKMS ANQVKDVFRF
IDNDQSGYLD
EEELKFFLQK FESGARELTE SETKSLMAAA DNDGDGKIGA
EEFQEMVHS
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UCSC
uc003spe.5; human.;
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