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Alternative name
Orexin ELISA KIT; Hypocretin ELISA KIT; HcrtHCRT ELISA KIT; OX ELISA KIT; PPORX ELISA KIT; PPOX ELISA KIT; Hcrt ELISA KIT; Hcrt1 ELISA KIT; Hcrt2 ELISA KIT
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Catalog
E027746
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species
Human
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GeneOX A
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL.
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Intended UseHuman OX A ELISA Kit allows for the in vitro quantitative determination of OX A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyNeurobiology
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Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This OX-A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human OX-A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY OX-A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OX-A antibody and an OX-A-HRP conjugate. The assay sample and buffer are incubated together with OX-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OX-A concentration since OX-A from samples and OX-A-HRP conjugate compete for the anti-OX-A antibody binding site. Since the number of sites is limited, as more sites are occupied by OX-A from the sample, fewer sites are left to bind OX-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OX-A concentration in each sample is interpolated from this standard curve.
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