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Alternative name
Osteopontin ELISA KIT; Bone sialoprotein 1 ELISA KIT; Nephropontin ELISA KIT; Secreted phosphoprotein 1 ELISA KIT; SPP-1 ELISA KIT; Urinary stone protein ELISA KIT; UropontinSPP1 ELISA KIT; BNSP ELISA KIT; OPN ELISA KIT; SPP-1 ELISA KIT
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Catalog
E027865
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species
Humanman
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GeneSPP1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHumanman SPP1 ELISA Kit allows for the in vitro quantitative determination of SPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The Human Osteopontin ELISA is a sandwich type immunoassay. The 96-well microplate is coated with a polyclonal antibody specific for human osteopontin. The standards, controls, and samples are added to the microplate wells. The microplate is then incubated at room temperature without agitation. After the first incubation is complete, the wells are washed with Wash Buffer and blotted dry. The polyclonal detection antibody conjugate is then added, and the microplate is incubated a second time at room temperature without agitation. After the second incubation is complete, the wells are washed with Wash Buffer and blotted dry. HRP conjugated streptavidin is then added, and the microplate is incubated a third time. After the third incubation is complete, the wells are washed with Wash Buffer and blotted dry. TMB Substrate is added, and the microplate is incubated a fourth time at room temperature without agitation. Once the incubation is complete, Stop Solution is added, and the optical density (OD) is measured by a spectrophotometer at 450 nm. The intensity of the color generated is directly proportional to the amount of human osteopontin in the sample.
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