Human PAH ELISA Kit

Alternative name
Human PAH ELISA Kit;Human phe-4-monooxygenase ELISA Kit;Human PH ELISA Kit;Human PKU ELISA Kit;Human PKU1 ELISA Kit;Human phenylalanine hydroxylase ELISA Kit;Human phenylalanine-4-hydroxylase ELISA Kit;Human phenylalanine 4-monooxygenase ELISA Kit;
Catalog
E028110
species
Human
GenePAH
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PAH. No significant cross-reactivity or interference between Human PAH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PAH and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity46.875pg/mL
Intended UseHuman PAH ELISA Kit allows for the in vitro quantitative determination of PAH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PAH in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PAH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PAH. You can calculate the concentration of Human PAH in the samples by comparing the OD of the samples to the standard curve.
Human Phenylalanine-4-hydroxylase Protein information
Uniprot ID PH4H_HUMAN
Uniprot AC P00439; Q16717; Q8TC14;
UniGene Hs.603740;
GeneID 5053
KEGG hsa:5053;
Human Phenylalanine-4-hydroxylase Protein SEQUENCE
SEQUENCE 452 AA; 51862 MW; 018F00EBBBDDCE2F CRC64;
MSTAVLENPG LGRKLSDFGQ ETSYIEDNCN QNGAISLIFS LKEEVGALAK
VLRLFEENDV NLTHIESRPS RLKKDEYEFF THLDKRSLPA LTNIIKILRH
DIGATVHELS RDKKKDTVPW FPRTIQELDR FANQILSYGA ELDADHPGFK
DPVYRARRKQ FADIAYNYRH GQPIPRVEYM EEEKKTWGTV FKTLKSLYKT
HACYEYNHIF PLLEKYCGFH EDNIPQLEDV SQFLQTCTGF RLRPVAGLLS
SRDFLGGLAF RVFHCTQYIR HGSKPMYTPE PDICHELLGH VPLFSDRSFA
QFSQEIGLAS LGAPDEYIEK LATIYWFTVE FGLCKQGDSI KAYGAGLLSS
FGELQYCLSE KPKLLPLELE KTAIQNYTVT EFQPLYYVAE SFNDAKEKVR
NFAATIPRPF SVRYDPYTQR IEVLDNTQQL KILADSINSE IGILCSALQK
IK
UCSC uc001tjq.2; human.;

Category

Human PAH ELISA Kit

Human PAH ELISA Kit;Human phe-4-monooxygenase ELISA Kit;Human PH ELISA Kit;Human PKU ELISA Kit;Human PKU1 ELISA Kit;Human phenylalanine hydroxylase ELISA Kit;Human phenylalanine-4-hydroxylase ELISA Kit;Human phenylalanine 4-monooxygenase ELISA Kit;