Human PAX9 ELISA Kit

Alternative name
Human STHAG3 ELISA Kit;Human paired box 9 ELISA Kit;Human paired box protein Pax-9 ELISA Kit;Human paired domain gene 9 ELISA Kit;
Catalog
E028126
species
Human
GenePAX9
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman PAX9 ELISA Kit allows for the in vitro quantitative determination of PAX9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: PAX9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAX9 antibody and an PAX9-HRP conjugate. The assay sample and buffer are incubated together with PAX9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAX9 concentration since PAX9 from samples and PAX9-HRP conjugate compete for the anti-PAX9 antibody binding site. Since the number of sites is limited, as more sites are occupied by PAX9 from the sample, fewer sites are left to bind PAX9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAX9 concentration in each sample is interpolated from this standard curve.
Human Paired box protein Pax-9 Protein information
Uniprot ID PAX9_HUMAN
Uniprot AC P55771; Q99582; Q9UQR4;
UniGene Hs.132576; Hs.609574;
GeneID 5083
KEGG hsa:5083;
Human Paired box protein Pax-9 Protein SEQUENCE
SEQUENCE 341 AA; 36310 MW; F5E6B0BC991E7C1D CRC64;
MEPAFGEVNQ LGGVFVNGRP LPNAIRLRIV ELAQLGIRPC DISRQLRVSH
GCVSKILARY NETGSILPGA IGGSKPRVTT PTVVKHIRTY KQRDPGIFAW
EIRDRLLADG VCDKYNVPSV SSISRILRNK IGNLAQQGHY DSYKQHQPTP
QPALPYNHIY SYPSPITAAA AKVPTPPGVP AIPGSVAMPR TWPSSHSVTD
ILGIRSITDQ VSDSSPYHSP KVEEWSSLGR NNFPAAAPHA VNGLEKGALE
QEAKYGQAPN GLPAVGSFVS ASSMAPYPTP AQVSPYMTYS AAPSGYVAGH
GWQHAGGTSL SPHNCDIPAS LAFKGMQAAR EGSHSVTASA L

Category

Human PAX9 ELISA Kit

Human STHAG3 ELISA Kit;Human paired box 9 ELISA Kit;Human paired box protein Pax-9 ELISA Kit;Human paired domain gene 9 ELISA Kit;