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Human PITX1 ELISA Kit

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  • Alternative name

    Human Hindlimb-expressed homeobox protein backfoot ELISA Kit;Human homeobox protein PITX1 ELISA Kit;Human paired-like homeodomain transcription factor 1 ELISA Kit;Human BFT ELISA Kit;Human PTX1 ELISA Kit;Human CCF ELISA Kit;Human LBNBG ELISA Kit;Human POTX ELISA Kit;Human paired like homeodomain 1 ELISA Kit;Human pituitary homeobox 1 ELISA Kit;Human hindlimb expressed homeobox protein backfoot ELISA Kit;Human pituitary homeo box 1 ELISA Kit;Human pituitary otx-related factor ELISA Kit;
  • Catalog
    E028138
  • species
    Human
  • GenePITX1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PITX1. No significant cross-reactivity or interference between human PITX1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PITX1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman PITX1 ELISA Kit allows for the in vitro quantitative determination of PITX1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PITX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PITX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PITX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PITX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Pituitary homeobox 1 Protein information
  • Uniprot ID PITX1_HUMAN
  • Uniprot AC P78337; A8K3M0; D3DQB0; O14677; O60425; Q9BTI5;
  • UniGene Hs.84136;
  • GeneID 5307
  • KEGG hsa:5307;
  • Human Pituitary homeobox 1 Protein SEQUENCE

  • SEQUENCE 314 AA; 34128 MW; F0DFE45E14508A43 CRC64;

    MDAFKGGMSL ERLPEGLRPP PPPPHDMGPA FHLARPADPR EPLENSASES

    SDTELPEKER GGEPKGPEDS GAGGTGCGGA DDPAKKKKQR RQRTHFTSQQ

    LQELEATFQR NRYPDMSMRE EIAVWTNLTE PRVRVWFKNR RAKWRKRERN

    QQLDLCKGGY VPQFSGLVQP YEDVYAAGYS YNNWAAKSLA PAPLSTKSFT

    FFNSMSPLSS QSMFSAPSSI SSMTMPSSMG PGAVPGMPNS GLNNINNLTG

    SSLNSAMSPG ACPYGTPASP YSVYRDTCNS SLASLRLKSK QHSSFGYGGL

    QGPASGLNAC QYNS




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