Human PTF1A ELISA Kit

Alternative name
Human class A basic helix-loop-helix protein 29 ELISA Kit;Human bHLHa29 ELISA Kit;Human Pancreas-specific transcription factor 1a ELISA Kit;Human bHLH transcription factor p48 ELISA Kit;Human p48 DNA-binding subunit of transcription factor PTF1 ELISA Kit;Human PTF1-p48 ELISA Kit;Human PTF1P48 ELISA Kit;Human PACA ELISA Kit;Human PAGEN2 ELISA Kit;Human pancreas specific transcription factor, 1a ELISA Kit;Human pancreas transcription factor 1 subunit alpha ELISA Kit;Human class II bHLH protein PTF1A ELISA Kit;Human exocrine pancreas-specific transcription factor p48 ELISA Kit;
Catalog
E028161
species
Human
GenePTF1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pancreas Specific Transcription Factor 1a (PTF1a). No significant cross-reactivity or interference between Pancreas Specific Transcription Factor 1a (PTF1a) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman PTF1A ELISA Kit allows for the in vitro quantitative determination of PTF1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pancreas Specific Transcription Factor 1a (PTF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pancreas Specific Transcription Factor 1a (PTF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pancreas Specific Transcription Factor 1a (PTF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreas Specific Transcription Factor 1a (PTF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Pancreas transcription factor 1 subunit alpha Protein information
Uniprot ID PTF1A_HUMAN
Uniprot AC Q7RTS3; Q9HC25;
UniGene Hs.351503;
GeneID 256297
KEGG hsa:256297;
Human Pancreas transcription factor 1 subunit alpha Protein SEQUENCE
SEQUENCE 328 AA; 34970 MW; 5AC84B482C55CEC3 CRC64;
MDAVLLEHFP GGLDAFPSSY FDEDDFFTDQ SSRDPLEDGD ELLADEQAEV
EFLSHQLHEY CYRDGACLLL QPAPPAAPLA LAPPSSGGLG EPDDGGGGGY
CCETGAPPGG FPYSPGSPPS CLAYPCAGAA VLSPGARLRG LSGAAAAAAR
RRRRVRSEAE LQQLRQAANV RERRRMQSIN DAFEGLRSHI PTLPYEKRLS
KVDTLRLAIG YINFLSELVQ ADLPLRGGGA GGCGGPGGGG RLGGDSPGSQ
AQKVIICHRG TRSPSPSDPD YGLPPLAGHS LSWTDEKQLK EQNIIRTAKV
WTPEDPRKLN SKSSFNNIEN EPPFEFVS
UCSC uc001irp.4; human.;

Category

Human PTF1A ELISA Kit