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Alternative name
Pancreatic prohormone ELISA KIT; Pancreatic polypeptide ELISA KIT; PPPPY ELISA KIT; PNP ELISA KIT; PP ELISA KIT; PH ELISA KIT; PI ELISA KIT
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Catalog
E028191
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species
Human
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GenePP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pancreatic Polypeptide (PP).
No significant cross-reactivity or interference between Pancreatic Polypeptide (PP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 13.8pg/mL.
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Intended UseHuman PP ELISA Kit allows for the in vitro quantitative determination of PP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Pancreatic Polypeptide (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Pancreatic Polypeptide (PP) and unlabeled Pancreatic Polypeptide (PP) (Standards or samples) with the pre-coated antibody specific to Pancreatic Polypeptide (PP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Pancreatic Polypeptide (PP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Pancreatic Polypeptide (PP) in the sample.
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