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Human PPY ELISA Kit

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  • Alternative name

    Human pancreatic polypeptide ELISA Kit;Human PP ELISA Kit;Human PH ELISA Kit;Human PI ELISA Kit;Human PNP ELISA Kit;Human pancreatic prohormone ELISA Kit;Human pancreatic polypeptide Y ELISA Kit;Human prepro-PP (prepropancreatic polypeptide) ELISA Kit;
  • Catalog
    E028195
  • species
    Human
  • GenePPY
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<28.1pg/ml
  • Intended UseHuman PPY ELISA Kit allows for the in vitro quantitative determination of PPY , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PPY antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PPY antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PPY amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PPY can be calculated.
  • Human Pancreatic icosapeptide Protein information
  • Uniprot ID PAHO_HUMAN
  • Uniprot AC P01298;
  • UniGene Hs.558368; Hs.744427;
  • GeneID 5539
  • KEGG hsa:5539;
  • Human Pancreatic icosapeptide Protein SEQUENCE

  • SEQUENCE 95 AA; 10445 MW; 44F0265092F9C4A0 CRC64;

    MAAARLCLSL LLLSTCVALL LQPLLGAQGA PLEPVYPGDN ATPEQMAQYA

    ADLRRYINML TRPRYGKRHK EDTLAFSEWG SPHAAVPREL SPLDL

  • UCSC uc002iep.4; human. [P01298-1];



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