Human PON2 ELISA Kit

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  • Alternative name

    Human PON 2 ELISA Kit;Human aromatic esterase 2 ELISA Kit;Human A-esterase 2 ELISA Kit;Human serum aryldialkylphosphatase 2 ELISA Kit;Human paraoxonase 2 ELISA Kit;Human serum paraoxonase/arylesterase 2 ELISA Kit;Human arylesterase 2 ELISA Kit;Human paraoxonase nirs ELISA Kit;

  • Catalog
    E028301
  • species
    Human
  • GenePON2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Paraoxonase 2 (PON2). No significant cross-reactivity or interference between Paraoxonase 2 (PON2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseHuman PON2 ELISA Kit allows for the in vitro quantitative determination of PON2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paraoxonase 2 (PON2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paraoxonase 2 (PON2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paraoxonase 2 (PON2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paraoxonase 2 (PON2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Serum paraoxonase/arylesterase 2 Protein information
  • Uniprot ID PON2_HUMAN
  • Uniprot AC Q86YL0;
  • UniGene Hs.514420; Hs.744912;
  • GeneID 5445
  • KEGG hsa:5445;
  • Human Serum paraoxonase/arylesterase 2 Protein SEQUENCE
  • SEQUENCE 354 AA; 39397 MW; 05CEDC84D5E7EC2A CRC64;

    MGRLVAVGLL GIALALLGER LLALRNRLKA SREVESVDLP HCHLIKGIEA

    GSEDIDILPN GLAFFSVGLK FPGLHSFAPD KPGGILMMDL KEEKPRAREL

    RISRGFDLAS FNPHGISTFI DNDDTVYLFV VNHPEFKNTV EIFKFEEAEN

    SLLHLKTVKH ELLPSVNDIT AVGPAHFYAT NDHYFSDPFL KYLETYLNLH

    WANVVYYSPN EVKVVAEGFD SANGINISPD DKYIYVADIL AHEIHVLEKH

    TNMNLTQLKV LELDTLVDNL SIDPSSGDIW VGCHPNGQKL FVYDPNNPPS

    SEVLRIQNIL CEKPTVTTVY ANNGSVLQGS SVASVYDGKL LIGTLYHRAL

    YCEL

  • UCSC uc003unv.4; human. [Q15165-2];



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