Human PARK2 ELISA Kit

Alternative name
Human Parkin ELISA Kit;Human parkinson juvenile disease protein 2 ELISA Kit;Human Parkinson disease protein 2 ELISA Kit;Human PRKN ELISA Kit;Human AR-JP ELISA Kit;Human LPRS2 ELISA Kit;Human PDJ ELISA Kit;Human parkin RBR E3 ubiquitin protein ligase ELISA Kit;Human E3 ubiquitin-protein ligase parkin ELISA Kit;Human Parkinson disease (autosomal recessive, juvenile) 2, parkin ELISA Kit;Human parkinson protein 2 E3 ubiquitin protein ligase ELISA Kit;Human parkinson protein 2, E3 ubiquitin protein ligase (parkin) ELISA Kit;
Catalog
E028336
species
Human
GenePARK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of PARK2. No significant cross-reactivity or interference between PARK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PARK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0pg/mL.
Intended UseHuman PARK2 ELISA Kit allows for the in vitro quantitative determination of PARK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyHuman ELISA Kit
Product Description
specificalIntended Uses: This PARK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PARK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PARK2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PARK2. Standards or samples are then added to the microtiter plate wells and PARK2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PARK2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PARK2 are added to each well to "sandwich" the PARK2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PARK2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PARK2 concentration in each sample is interpolated from this standard curve.
Human E3 ubiquitin-protein ligase parkin Protein information
Uniprot ID PRKN2_HUMAN
Uniprot AC Q8NI41; Q8NI43; Q8NI44; Q8WW07;
UniGene Hs.132954;
GeneID 5071
KEGG hsa:5071;
Human E3 ubiquitin-protein ligase parkin Protein SEQUENCE
SEQUENCE 465 AA; 51641 MW; 9A8BB802A3FC84C3 CRC64;
MIVFVRFNSS HGFPVEVDSD TSIFQLKEVV AKRQGVPADQ LRVIFAGKEL
RNDWTVQNCD LDQQSIVHIV QRPWRKGQEM NATGGDDPRN AAGGCEREPQ
SLTRVDLSSS VLPGDSVGLA VILHTDSRKD SPPAGSPAGR SIYNSFYVYC
KGPCQRVQPG KLRVQCSTCR QATLTLTQGP SCWDDVLIPN RMSGECQSPH
CPGTSAEFFF KCGAHPTSDK ETSVALHLIA TNSRNITCIT CTDVRSPVLV
FQCNSRHVIC LDCFHLYCVT RLNDRQFVHD PQLGYSLPCV AGCPNSLIKE
LHHFRILGEE QYNRYQQYGA EECVLQMGGV LCPRPGCGAG LLPEPDQRKV
TCEGGNGLGC GFAFCRECKE AYHEGECSAV FEASGTTTQA YRVDERAAEQ
ARWEAASKET IKKTTKPCPR CHVPVEKNGG CMHMKCPQPQ CRLEWCWNCG
CEWNRVCMGD HWFDV
UCSC uc003qty.5; human. [O60260-1];

Category

Human PARK2 ELISA Kit