Human PTCH1 ELISA Kit

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  • Alternative name

    Human PTC ELISA Kit;Human PTC1 ELISA Kit;Human PTCH ELISA Kit;Human BCNS ELISA Kit;Human HPE7 ELISA Kit;Human NBCCS ELISA Kit;Human PTCH11 ELISA Kit;Human patched 1 ELISA Kit;Human protein patched homolog 1 ELISA Kit;Human PTCH protein +12b ELISA Kit;Human PTCH protein +4' ELISA Kit;Human PTCH protein -10 ELISA Kit;Human PTCH protein -3,4,5 ELISA Kit;

  • Catalog
    E028366
  • species
    Human
  • GenePTCH1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PTCH1. No significant cross-reactivity or interference between human PTCH1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PTCH1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman PTCH1 ELISA Kit allows for the in vitro quantitative determination of PTCH1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTCH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTCH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTCH1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTCH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein patched homolog 1 Protein information
  • Uniprot ID PTC1_HUMAN
  • Uniprot AC Q5VZC2; Q86XG7;
  • UniGene Hs.494538;
  • GeneID 5727
  • KEGG hsa:5727;
  • Human Protein patched homolog 1 Protein SEQUENCE
  • SEQUENCE 1447 AA; 160545 MW; F2937247BC812F85 CRC64;

    MASAGNAAEP QDRGGGGSGC IGAPGRPAGG GRRRRTGGLR RAAAPDRDYL

    HRPSYCDAAF ALEQISKGKA TGRKAPLWLR AKFQRLLFKL GCYIQKNCGK

    FLVVGLLIFG AFAVGLKAAN LETNVEELWV EVGGRVSREL NYTRQKIGEE

    AMFNPQLMIQ TPKEEGANVL TTEALLQHLD SALQASRVHV YMYNRQWKLE

    HLCYKSGELI TETGYMDQII EYLYPCLIIT PLDCFWEGAK LQSGTAYLLG

    KPPLRWTNFD PLEFLEELKK INYQVDSWEE MLNKAEVGHG YMDRPCLNPA

    DPDCPATAPN KNSTKPLDMA LVLNGGCHGL SRKYMHWQEE LIVGGTVKNS

    TGKLVSAHAL QTMFQLMTPK QMYEHFKGYE YVSHINWNED KAAAILEAWQ

    RTYVEVVHQS VAQNSTQKVL SFTTTTLDDI LKSFSDVSVI RVASGYLLML

    AYACLTMLRW DCSKSQGAVG LAGVLLVALS VAAGLGLCSL IGISFNAATT

    QVLPFLALGV GVDDVFLLAH AFSETGQNKR IPFEDRTGEC LKRTGASVAL

    TSISNVTAFF MAALIPIPAL RAFSLQAAVV VVFNFAMVLL IFPAILSMDL

    YRREDRRLDI FCCFTSPCVS RVIQVEPQAY TDTHDNTRYS PPPPYSSHSF

    AHETQITMQS TVQLRTEYDP HTHVYYTTAE PRSEISVQPV TVTQDTLSCQ

    SPESTSSTRD LLSQFSDSSL HCLEPPCTKW TLSSFAEKHY APFLLKPKAK

    VVVIFLFLGL LGVSLYGTTR VRDGLDLTDI VPRETREYDF IAAQFKYFSF

    YNMYIVTQKA DYPNIQHLLY DLHRSFSNVK YVMLEENKQL PKMWLHYFRD

    WLQGLQDAFD SDWETGKIMP NNYKNGSDDG VLAYKLLVQT GSRDKPIDIS

    QLTKQRLVDA DGIINPSAFY IYLTAWVSND PVAYAASQAN IRPHRPEWVH

    DKADYMPETR LRIPAAEPIE YAQFPFYLNG LRDTSDFVEA IEKVRTICSN

    YTSLGLSSYP NGYPFLFWEQ YIGLRHWLLL FISVVLACTF LVCAVFLLNP

    WTAGIIVMVL ALMTVELFGM MGLIGIKLSA VPVVILIASV GIGVEFTVHV

    ALAFLTAIGD KNRRAVLALE HMFAPVLDGA VSTLLGVLML AGSEFDFIVR

    YFFAVLAILT ILGVLNGLVL LPVLLSFFGP YPEVSPANGL NRLPTPSPEP

    PPSVVRFAMP PGHTHSGSDS SDSEYSSQTT VSGLSEELRH YEAQQGAGGP

    AHQVIVEATE NPVFAHSTVV HPESRHHPPS NPRQQPHLDS GSLPPGRQGQ

    QPRRDPPREG LWPPPYRPRR DAFEISTEGH SGPSNRARWG PRGARSHNPR

    NPASTAMGSS VPGYCQPITT VTASASVTVA VHPPPVPGPG RNPRGGLCPG

    YPETDHGLFE DPHVPFHVRC ERRDSKVEVI ELQDVECEER PRGSSSN

  • UCSC uc004avk.5; human. [Q13635-1];



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