Human PEPD ELISA Kit

Alternative name
Human X-Pro dipeptidase ELISA Kit;Human imidodipeptidase ELISA Kit;Human peptidase D ELISA Kit;Human proline dipeptidase ELISA Kit;Human PROLIDASE ELISA Kit;Human PRD ELISA Kit;Human xaa-Pro dipeptidase ELISA Kit;Human aminoacyl-L-proline hydrolase ELISA Kit;Human testicular tissue protein Li 138 ELISA Kit;
Catalog
E028526
species
Human
GenePEPD
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidase D (PEPD). No significant cross-reactivity or interference between Peptidase D (PEPD) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.63ng/mL.
Intended UseHuman PEPD ELISA Kit allows for the in vitro quantitative determination of PEPD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidase D (PEPD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidase D (PEPD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidase D (PEPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidase D (PEPD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Xaa-Pro dipeptidase Protein information
Uniprot ID PEPD_HUMAN
Uniprot AC Q8TBN9; Q9BT75;
UniGene Hs.36473;
GeneID 5184
KEGG hsa:5184;
Human Xaa-Pro dipeptidase Protein SEQUENCE
SEQUENCE 493 AA; 54548 MW; E8C4A2497E44BA22 CRC64;
MAAATGPSFW LGNETLKVPL ALFALNRQRL CERLRKNPAV QAGSIVVLQG
GEETQRYCTD TGVLFRQESF FHWAFGVTEP GCYGVIDVDT GKSTLFVPRL
PASHATWMGK IHSKEHFKEK YAVDDVQYVD EIASVLTSQK PSVLLTLRGV
NTDSGSVCRE ASFDGISKFE VNNTILHPEI VECRVFKTDM ELEVLRYTNK
ISSEAHREVM KAVKVGMKEY ELESLFEHYC YSRGGMRHSS YTCICGSGEN
SAVLHYGHAG APNDRTIQNG DMCLFDMGGE YYCFASDITC SFPANGKFTA
DQKAVYEAVL RSSRAVMGAM KPGVWWPDMH RLADRIHLEE LAHMGILSGS
VDAMVQAHLG AVFMPHGLGH FLGIDVHDVG GYPEGVERID EPGLRSLRTA
RHLQPGMVLT VEPGIYFIDH LLDEALADPA RASFLNREVL QRFRGFGGVR
IEEDVVVTDS GIELLTCVPR TVEEIEACMA GCDKAFTPFS GPK
UCSC uc002nur.5; human. [P12955-1];

Category

Human PEPD ELISA Kit